Microfluidic Organ-on-a-Chip Models of Human Intestine

被引：444

作者：

Bein, Amir ^{[1
]}

Shin, Woojung ^{[2
]}

Jalili-Firoozinezhad, Sasan ^{[1
,3
,4
]}

Park, Min Hee ^{[2
]}

Sontheimer-Phelps, Alexandra ^{[1
,5
]}

Tovaglieri, Alessio ^{[1
,6
]}

Chalkiadaki, Angeliki ^{[1
]}

Kim, Hyun Jung ^{[2
]}

Ingber, Donald E. ^{[1
,7
,8
,9
,10
]}

机构：

[1] Harvard Univ, Wyss Inst Biol Inspired Engn, CLSB Bldg,5th Floor,3 Blackfan Circle, Boston, MA 02115 USA

[2] Univ Texas Austin, Dept Biomed Engn, 107 West Dean Keeton St,BME 4-202C, Austin, TX 78712 USA

[3] Univ Lisbon, Inst Super Tecn, Dept Bioengn, Lisbon, Portugal

[4] Univ Lisbon, Inst Super Tecn, IBB, Lisbon, Portugal

[5] Univ Freiburg, Fac Biol, Grad Program, Freiburg, Germany

[6] Swiss Fed Inst Technol, Dept Hlth Sci & Technol, Grad Program, Zurich, Switzerland

[7] Boston Childrens Hosp, Vasc Biol Program, Boston, MA USA

[8] Boston Childrens Hosp, Dept Surg, Boston, MA USA

[9] Harvard Med Sch, Boston, MA USA

[10] Harvard John A Paulson Sch Engn & Appl Sci, Cambridge, MA USA

来源：

CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY | 2018年 / 5卷 / 04期

关键词：

Organs-on-Chips; Gut-on-a-Chip; Intestine-on-a-Chip; Microfluidic; IN-VITRO MODEL; CLOSTRIDIUM-DIFFICILE INFECTION; PLURIPOTENT STEM-CELL; CHAIN FATTY-ACIDS; NERVOUS-SYSTEM; BACTERIAL OVERGROWTH; DRUG PERMEABILITY; GUT MICROBIOTA; USSING CHAMBER; CULTURE;

D O I：

10.1016/j.jcmgh.2017.12.010

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Organs-on-chips are microfluidic cell culture systems that recapitulate the structure, function, physiology, and pathology of living human organs in vitro. In this article, we review recent development of various human intestine-on-a-chip models and their potential value for disease modeling, drug discovery, and personalized medicine. Microfluidic organ-on-a- chip models of human intestine have been developed and used to study intestinal physiology and pathophysiology. In this article, we review this field and describe how microfluidic Intestine Chips offer new capabilities not possible with conventional culture systems or organoid cultures, including the ability to analyze contributions of individual cellular, chemical, and physical control parameters one-at-a-time; to coculture human intestinal cells with commensal microbiome for extended times; and to create human-relevant disease models. We also discuss potential future applications of human Intestine Chips, including how they might be used for drug development and personalized medicine.

引用

页码：659 / 668

页数：10

共 101 条

[1] Orai1-Mediated Antimicrobial Secretion from Pancreatic Acini Shapes the Gut Microbiome and Regulates Gut Innate Immunity [J].

Ahuja, Malini ;

Schwartz, Daniella M. ;

Tandon, Mayank ;

Son, Aran ;

Zeng, Mei ;

Swaim, William ;

Eckhaus, Michael ;

Hoffman, Victoria ;

Cui, Yiyuan ;

Xiao, Bo ;

Worley, Paul F. ;

Muallem, Shmuel .

CELL METABOLISM, 2017, 25 (03) :635-646

[2] Everted gut sac model as a tool in pharmaceutical research: limitations and applications [J].

Alam, Mohd Aftab ;

Al-Jenoobi, Fahad Ibrahim ;

Al-mohizea, Abdullah M. .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2012, 64 (03) :326-336

[3] CORRELATION BETWEEN ORAL-DRUG ABSORPTION IN HUMANS AND APPARENT DRUG PERMEABILITY COEFFICIENTS IN HUMAN INTESTINAL EPITHELIAL (CACO-2) CELLS [J].

ARTURSSON, P ;

KARLSSON, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 175 (03) :880-885

[4] Micro-dissected tumor tissues on chip: an ex vivo method for drug testing and personalized therapy [J].

Astolfi, M. ;

Peant, B. ;

Lateef, M. A. ;

Rousset, N. ;

Kendall-Dupont, J. ;

Carmona, E. ;

Monet, F. ;

Saad, F. ;

Provencher, D. ;

Mes-Masson, A. -M. ;

Gervais, T. .

LAB ON A CHIP, 2016, 16 (02) :312-325

[5] Treating Clostridium difficile Infection With Fecal Microbiota Transplantation [J].

Bakken, Johan S. ;

Borody, Thomas ;

Brandt, Lawrence J. ;

Brill, Joel V. ;

Demarco, Daniel C. ;

Franzos, Marc Alaric ;

Kelly, Colleen ;

Khoruts, Alexander ;

Louie, Thomas ;

Martinelli, Lawrence P. ;

Moore, Thomas A. ;

Russell, George ;

Surawicz, Christina .

CLINICAL GASTROENTEROLOGY AND HEPATOLOGY, 2011, 9 (12) :1044-1049

[6]

Benam KH, 2016, NAT METHODS, V13, P151, DOI [10.1038/NMETH.3697, 10.1038/nmeth.3697]

[7] Intestinal drug metabolism and antitransport processes: A potential paradigm shift in oral drug delivery [J].

Benet, LZ ;

Wu, CY ;

Hebert, MF ;

Wacher, VJ .

JOURNAL OF CONTROLLED RELEASE, 1996, 39 (2-3) :139-143

[8] Microfluidic organs-on-chips [J].

Bhatia, Sangeeta N. ;

Ingber, Donald E. .

NATURE BIOTECHNOLOGY, 2014, 32 (08) :760-772

[9] Short chain fatty acids exchange across the gut and liver in humans measured at surgery [J].

Bloemen, Johanne G. ;

Venema, Koen ;

de Poll, Marcel C. van ;

Damink, Steven W. Olde ;

Buurman, Wim A. ;

Dejong, Cornelis H. .

CLINICAL NUTRITION, 2009, 28 (06) :657-661

[10] Diet and specific microbial exposure trigger features of environmental enteropathy in a novel murine model [J].

Brown, Eric M. ;

Wlodarska, Marta ;

Willing, Benjamin P. ;

Vonaesch, Pascale ;

Han, Jun ;

Reynolds, Lisa A. ;

Arrieta, Marie-Claire ;

Uhrig, Marco ;

Scholz, Roland ;

Partida, Oswaldo ;

Borchers, Christoph H. ;

Sansonetti, Philippe J. ;

Finlay, B. Brett .

NATURE COMMUNICATIONS, 2015, 6

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