Annexin A1 subcellular expression in laryngeal squamous cell carcinoma

被引：24

作者：

Alves, V. A. F. ^{[2
]}

Nonogaki, S. ^{[3
]}

Cury, P. M. ^{[4
]}

Wuensch-Filho, V. ^{[5
]}

de Carvalho, M. B. ^{[6
]}

Michaluart-Junior, P. ^{[7
]}

Moyses, R. A. ^{[7
]}

Curioni, O. A. ^{[6
]}

Figueiredo, D. L. A. ^{[8
]}

Scapulatempo-Neto, C. ^{[2
]}

Parra, E. R. ^{[2
]}

Polachini, G. M. ^{[1
]}

Silistino-Souza, R. ^{[9
]}

Oliani, S. M. ^{[9
]}

Silva-Junior, W. A. ^{[10
]}

Nobrega, F. G. ^{[11
]}

Tajara, E. H. ^{[1
,12
]}

Zago, M. A. ^{[13
]}

机构：

[1] FAMERP, Sch Med, Dept Mol Biol, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil

[2] Univ Sao Paulo, Sch Med, Dept Pathol, BR-09500900 Sao Paulo, Brazil

[3] Adolfo Lutz Inst, Div Pathol, Sao Paulo, Brazil

[4] FAMERP, Sch Med, Dept Pathol, BR-15090000 Sao Jose Do Rio Preto, SP, Brazil

[5] Univ Sao Paulo, Sch Publ Hlth, Dept Epidemiol, BR-09500900 Sao Paulo, Brazil

[6] Heliopolis Hosp, Dept Head & Neck Surg, Sao Paulo, Brazil

[7] Univ Sao Paulo, Sch Med, Dept Surg, Div Head & Neck Surg, BR-09500900 Sao Paulo, Brazil

[8] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Ophthalmol Otorhinolaryngol & Head & Neck Su, Sao Jose Do Rio Preto, Brazil

[9] UNESP, Sao Paulo State Univ, IBILCE,Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, Brazil

[10] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Genet, Sao Jose Dos Campos, Brazil

[11] UNESP, Sao Paulo State Univ, Sao Jose Dos Campos Dent Sch, Dept Biosci & Oral Diag, Sao Jose Dos Campos, Brazil

[12] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Sao Paulo, SP, Brazil

[13] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Clin Med, Sao Paulo, SP, Brazil

来源：

HISTOPATHOLOGY | 2008年 / 53卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

annexin A1; head and neck neoplasm; immunohistochemistry; laryngeal neoplasm;

D O I：

10.1111/j.1365-2559.2008.03186.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Annexin A1 (ANXA1) is a soluble cytoplasmic protein, moving to membranes when calcium levels are elevated. ANXA1 has also been shown to move to the nucleus or outside the cells, depending on tyrosine-kinase signalling, thus interfering in cytoskeletal organization and cell differentiation, mostly in inflammatory and neoplastic processes. The aim was to investigate subcellular patterns of immunohistochemical expression of ANXA1 in neoplastic and non-neoplastic samples from patients with laryngeal squamous cell carcinomas (LSCC), to elucidate the role of ANXA1 in laryngeal carcinogenesis. Serial analysis of gene expression experiments detected reduced expression of ANXA1 gene in LSCC compared with the corresponding non-neoplastic margins. Quantitative polymerase chain reaction confirmed ANXA1 low expression in 15 LSCC and eight matched normal samples. Thus, we investigated subcellular patterns of immunohistochemical expression of ANXA1 in 241 paraffin-embedded samples from 95 patients with LSCC. The results showed ANXA1 down-regulation in dysplastic, tumourous and metastatic lesions and provided evidence for the progressive migration of ANXA1 from the nucleus towards the membrane during laryngeal tumorigenesis. ANXA1 dysregulation was observed early in laryngeal carcinogenesis, in intra-epithelial neoplasms; it was not found related to prognostic parameters, such as nodal metastases.

引用

页码：715 / 727

页数：13

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