Rapid and sensitive detection of norovirus antibodies in human serum with a biolayer interferometry biosensor

被引：25
作者：
Auer, Sanna [1 ]
Koho, Tiia [1 ]
Uusi-Kerttula, Hanni [3 ]
Vesikari, Timo [4 ,5 ]
Blazevic, Vesna [5 ]
Hytonen, Vesa P. [2 ,6 ]
机构：
[1] Univ Tampere, BioMediTech, FIN-33101 Tampere, Finland
[2] Univ Tampere, BioMediTech, Prot Dynam Res Grp, FIN-33101 Tampere, Finland
[3] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland
[4] Univ Tampere, Sch Med, Virol, FIN-33101 Tampere, Finland
[5] Univ Tampere, Sch Med, Vaccine Res Ctr, FIN-33101 Tampere, Finland
[6] Fimlab Labs Ltd, Tampere, Finland
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2015年 / 221卷
基金：
芬兰科学院;
关键词：
Biolayer interferometry; Norovirus; Virus-like particles (VLPs); P-particles; Non-labelled detection; Fast diagnostics; VIRUS-LIKE PARTICLES; LABEL-FREE; IMMOBILIZATION; INFECTION; CHILDREN; PROTEIN; SURFACE; ASSAY; PCR;
D O I：
10.1016/j.snb.2015.06.088
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Here, we describe the use of a biolayer interferometry biosensor for the fast and sensitive detection of virus-specific antibodies from human serum samples. Norovirus-like particles and norovirus P-particles were used to functionalise the biosensor tip. The detection of antibodies directly from serum samples was challenging, but the addition of a metal chelator (DAB) combined with an anti-human horseradish peroxidase-tagged antibody enabled enhanced detection of virus-specific antibodies in serum dilutions up to 1:100,000. Biolayer interferometry provides results faster than an ELISA, with results in as little as 10-20 min when using pre-functionalised sensors. Therefore, biolayer interferometry combined with DAB enhancement offers an attractive method for quick and sensitive quantification of biomolecules from complicated sample matrices. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：507 / 514
页数：8
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