Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis

被引：8

作者：

Conter, Carolina Cella ^{[1
]}

Neitzke-Abreu, Herintha Coeto ^{[1
,2
]}

Pedroso, Raissa Bocchi ^{[3
]}

Campana Lonardoni, Maria Valdrinez ^{[1
,3
]}

Verzignassi Silveira, Thais Gomes ^{[1
,3
]}

Alessi Aristides, Sandra Mara ^{[1
,3
]}

机构：

[1] Univ Estadual Maringa, Programa Posgrad Ciencias Saude, Maringa, Parana, Brazil

[2] Fundacao Univ Fed Grande Dourados, Fac Ciencias Saude, Dourados, MS, Brazil

[3] Univ Estadual Maringa, Dept Anal Clin & Biomed, Maringa, Parana, Brazil

来源：

Revista da Sociedade Brasileira de Medicina Tropical | 2015年 / 48卷 / 05期

关键词：

Polymerase chain reaction; Leishmania (Viannia); Leucocyte; BRAZILIENSIS; INFECTIONS;

D O I：

10.1590/0037-8682-0052-2015

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Introduction: Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment. Methods: Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia). Results: DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively. Conclusions: The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance.

引用

页码：626 / 628

页数：3

共 15 条

[1] Haematogenous dissemination of Leishmania (Viannia) braziliensis in human American tegumentary leishmaniasis [J].

Camera, Patricia de Oliveira ;

Junger, Juliana ;

Santo Silva Piresa, Felipe do Espirito ;

Mattos, Marise ;

Oliveira-Neto, Manoel Paes ;

Fernandes, Octavio ;

Pirmez, Claude .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 2006, 100 (12) :1112-1117

[2] Some aspects of protozoan infections in immunocompromised patients - A review [J].

Ferreira, MS ;

Borges, AS .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (04) :443-457

[3] PRESENCE OF LEISHMANIA-BRAZILIENSIS IN BLOOD-SAMPLES FROM CURED PATIENTS OR AT DIFFERENT STAGES OF IMMUNOTHERAPY [J].

GUEVARA, P ;

ROJAS, E ;

GONZALEZ, N ;

SCORZA, JV ;

ANEZ, N ;

VALERA, M ;

RAMIREZ, JL .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1994, 1 (04) :385-389

[4] LEISHMANIA-BRAZILIENSIS IN BLOOD 30 YEARS AFTER CURE [J].

GUEVARA, P ;

RAMIREZ, JL ;

ROJAS, E ;

SCORZA, JV ;

GONZALEZ, N ;

ANEZ, N .

LANCET, 1993, 341 (8856) :1341-1341

[5]

Lima Airton Pereira, 2002, An. Bras. Dermatol., V77, P681, DOI 10.1590/S0365-05962002000600005

[6] DIAGNOSIS OF LEISHMANIA USING THE POLYMERASE CHAIN-REACTION - A SIMPLIFIED PROCEDURE FOR FIELD WORK [J].

LOPEZ, M ;

INGA, R ;

CANGALAYA, M ;

ECHEVARRIA, J ;

LLANOSCUENTAS, A ;

ORREGO, C ;

AREVALO, J .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1993, 49 (03) :348-356

[7]

Ministerio da Saude, 2013, MAN VIG LEISHM TEG A

[8] Detection of DNA from Leishmania (Viannia): Accuracy of Polymerase Chain Reaction for the Diagnosis of Cutaneous Leishmaniasis [J].

Neitzke-Abreu, Herintha Coeto ;

Venazzi, Mateus Sabaini ;

Zandonadi Bernal, Marcos Vinicius ;

Reinhold-Castro, Karin Rosi ;

Vagetti, Fernanda ;

Mota, Camila Alves ;

Silva, Naielly Rodrigues ;

Alessi Aristides, Sandra Mara ;

Verzignassi Silveira, Thais Gomes ;

Campana Lonardoni, Maria Valdrinez .

PLOS ONE, 2013, 8 (07)

[9] Persistent infections by Leishmania (Viannia) braziliensis [J].

Ramirez, JL ;

Guevara, P .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 1997, 92 (03) :333-338

[10] Viability and Burden of Leishmania in Extralesional Sites during Human Dermal Leishmaniasis [J].

Romero, Ibeth ;

Tellez, Jair ;

Suarez, Yazmin ;

Cardona, Maria ;

Figueroa, Roger ;

Zelazny, Adrian ;

Gore Saravia, Nancy .

PLOS NEGLECTED TROPICAL DISEASES, 2010, 4 (09)

← 1 2 →