Modulation of immune functions in polymorphonuclear neutrophils induced by physostigmine, but not neostigmine, independent of cholinergic neurons

被引：9

作者：

Bitzinger, Diane I. ^{[1
]}

Zausig, York A. ^{[1
]}

Paech, Christoph ^{[1
]}

Gruber, Michael ^{[1
]}

Busse, Hendrik ^{[1
]}

Sinner, Barbara ^{[1
]}

Graf, Bernhard M. ^{[1
]}

Trabold, Benedikt ^{[1
]}

机构：

[1] Univ Regensburg, Dept Anaesthesiol, D-93053 Regensburg, Germany

来源：

IMMUNOBIOLOGY | 2013年 / 218卷 / 08期

关键词：

Cholinergic-anti-inflammatory-pathway; Cholinesterase inhibitors; Immunomodulation; Mac-1; Non-neuronal-cholinergic system; Oxidative burst; Reactive oxygen species; SYSTEM; PHARMACOKINETICS; EXPRESSION;

D O I：

10.1016/j.imbio.2013.01.003

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Background: Cholinesterase inhibitors (Ch-I) improve survival in experimental sepsis consistent with activation of the cholinergic-anti-inflammatory-pathway. So far, less is known about whether Ch-I have a direct immunomodulatory effect on immune cells (polymorphonuclear neutrophils, PMN) in the absence of cholinergic neurons. We investigated the concentration-response-effects of physostigmine and neostigmine on the oxidative burst activity (human and rat PMN) and the expression of adhesion molecules on the surface of human PMN under in vitro conditions. Methods: PMN from 10 healthy humans or 10 rats were pretreated with 2, 10, 24, 97 mu M physostigmine or 3, 15, 30, 150 mu M neostigmine, primed with tumor-necrosis-factor-alpha (TNF-alpha) followed by stimulation with n-formyl-methionyl-leucylphenylalanine (fMLP) or stimulated with phorbol-12-myristate-13-acetate (PMA). Human and rat samples were assessed by flow cytometry for the generation of oxidative free radicals. Stimulated human PMN were additionally incubated with antibodies against Mac-1 (CD11b) or L-selectin (CD621). Results: Physostigmine and neostigmine did not alter oxidative burst activity or the expression of adhesion molecules of PMN induced by receptor-dependent activators like fMLP or TNF-alpha/fMLP (rat and human PMN, p = n.s.). Physostigmine, but not neostigmine, inhibited the protein-kinase-C-mediated oxidative burst activity by PMA in a dose-dependent manner (rat and human PMN, p < 0.05). Physostigmine, in the concentration range tested, suppressed the expression of CD11b following stimulation with PMA not significantly (human PMN: control: 63.1 +/- 10.7 vs. 97 physostigmine: 49.9 +/- 12.8 MESF, p = n.s.). Conclusion: While neostigmine has no effect on functional and phenotypic changes of PMN, the lipid soluble Ch-I physostigmine causes a dose dependent reduction in PMA-induced oxidative burst, independent of neuronal released acetylcholine. (C) 2013 Elsevier GmbH. All rights reserved.

引用

页码：1049 / 1054

页数：6

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