Influence of Particle Size and Antigen Binding on Effectiveness of Aluminum Salt Adjuvants in a Model Lysozyme Vaccine

被引：36

作者：

Clausi, Amber ^{[1
]}

Cummiskey, Jessica ^{[2
]}

Merkley, Scott ^{[1
]}

Carpenter, John F. ^{[2
]}

Braun, Latoya Jones ^{[2
]}

Randolph, Theodore W. ^{[1
]}

机构：

[1] Univ Colorado, Ctr Pharmaceut Biotechnol, Dept Chem & Biol Engn, Boulder, CO 80309 USA

[2] Univ Colorado, Hlth Sci Ctr, Dept Pharmaceut Sci, Denver, CO USA

来源：

JOURNAL OF PHARMACEUTICAL SCIENCES | 2008年 / 97卷 / 12期

关键词：

lysozyme; aluminum hydroxide; aluminum phosphate; adjuvant; particle size distribution;

D O I：

10.1002/jps.21390

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

It has been suggested that agglomeration of aluminum salt adjuvant particles during freezing and drying can cause loss of immunogenicity of vaccines formulated with such adjuvants. In this study, we tested this hypothesis and examined the immune response in a murine model to various liquid, freeze-thawed, and lyophilized vaccine formulations, using lysozyme as a model antigen. The various processing techniques and excipient levels resulted in a wide range of particle size distributions (PSDs) and antigen-adjuvant binding levels. Anti-lysozyme titers were independent of the PSD for vaccines adjuvanted with either aluminum hydroxide or aluminum phosphate and also were unaffected by the level of antigen binding to the adjuvant. (c) 2008 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 97:5252-5262, 2008

引用

页码：5252 / 5262

页数：11

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