Biobleaching of wheat straw pulp with recombinant laccase from the hyperthermophilic Thermus thermophilus

被引：25

作者：

Zheng, Zhiqiang ^{[3
]}

Li, Huazhong ^{[3
]}

Li, Lun ^{[2
]}

Shao, Weilan ^{[1
,3
,4
]}

机构：

[1] Nanjing Normal Univ, Res Ctr Biotechnol & Biomass Energy, Nanjing 210046, Jiangsu, Peoples R China

[2] Univ Sydney, Fac Vet Sci, Camden, NSW, Australia

[3] Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Peoples R China

[4] Nanjing Normal Univ, Jiangsu Key Lab Microbes & Funct Genom, Coll Life Sci, Nanjing 210046, Jiangsu, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 03期

关键词：

Biobleaching; Recombinant laccase; Thermus thermophilus; Wheat straw pulp; FUNGAL LACCASES; KRAFT PULP; SUBSTRATE;

D O I：

10.1007/s10529-011-0796-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90 degrees C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp kappa number was decreased by 5.6 U. Enzymatic treatment improved the bleachability of wheat straw pulp but caused no damage to the pulp fibers. The use of enzyme-treated pulp saved 25% H2O2 consumption in subsequent peroxide bleaching without decreasing the final brightness. Pulp biobleaching in the presence of 5 mM ABTS further increased the pulp brightness by 1.5% ISO. This is the first report on the application of laccase from T. thermophilus in the pulp and paper sector.

引用

页码：541 / 547

页数：7

共 17 条

[1] Kraft pulp biobleaching and mediated oxidation of a nonphenolic substrate by laccase from Streptomyces cyaneus CECT 3335 [J].

Arias, ME ;

Arenas, M ;

Rodríguez, J ;

Soliveri, J ;

Ball, AS ;

Hernández, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :1953-1958

[2] Promoting laccase activity towards non-phenolic substrates: a mechanistic investigation with some laccase-mediator systems [J].

Baiocco, P ;

Barreca, AM ;

Fabbrini, M ;

Galli, C ;

Gentili, P .

ORGANIC & BIOMOLECULAR CHEMISTRY, 2003, 1 (01) :191-197

[3] Application of enzymes in the pulp and paper industry [J].

Bajpai, P .

BIOTECHNOLOGY PROGRESS, 1999, 15 (02) :147-157

[4] Fungal laccases - occurrence and properties [J].

Baldrian, P .

FEMS MICROBIOLOGY REVIEWS, 2006, 30 (02) :215-242

[5] Laccases and their occurrence in prokaryotes [J].

Claus, H .

ARCHIVES OF MICROBIOLOGY, 2003, 179 (03) :145-150

[6] Industrial and biotechnological applications of laccases: A review [J].

Couto, Susana Rodriguez ;

Herrera, Jose Luis Toca .

BIOTECHNOLOGY ADVANCES, 2006, 24 (05) :500-513

[7] A robust metallo-oxidase from the hyperthermophilic bacterium Aquifex aeolicus [J].

Fernandes, Andre T. ;

Soares, Claudio M. ;

Pereira, Manuela M. ;

Huber, Robert ;

Grass, Gregor ;

Martins, Ligia O. .

FEBS JOURNAL, 2007, 274 (11) :2683-2694

[8] Thermotolerant and thermostable laccases [J].

Hilden, Kristiina ;

Hakala, Terhi K. ;

Lundell, Taina .

BIOTECHNOLOGY LETTERS, 2009, 31 (08) :1117-1128

[9] Molecular and biochemical characterization of a highly stable bacterial laccase that occurs as a structural component of the Bacillus subtilis endospore coat [J].

Martins, LO ;

Soares, CM ;

Pereira, MM ;

Teixeira, M ;

Costa, T ;

Jones, GH ;

Henriques, AO .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (21) :18849-18859

[10] A hyperthermophilic laccase from Thermus thermophilus HB27 [J].

Miyazaki, K .

EXTREMOPHILES, 2005, 9 (06) :415-425

← 1 2 →