Semen inhibits Zika virus infection of cells and tissues from the anogenital region

被引：37

作者：

Mueller, Janis A. ^{[1
]}

Harms, Mirja ^{[1
]}

Krueger, Franziska ^{[1
]}

Gross, Ruediger ^{[1
]}

Joas, Simone ^{[1
]}

Hayn, Manuel ^{[1
]}

Dietz, Andrea N. ^{[2
]}

Lippold, Sina ^{[2
]}

von Einem, Jens ^{[2
]}

Schubert, Axel ^{[2
]}

Michel, Manuela ^{[2
]}

Mayer, Benjamin ^{[3
]}

Cortese, Mirko ^{[4
]}

Jang, Karen S. ^{[5
,6
]}

Sandi-Monroy, Nathallie ^{[1
]}

Deniz, Miriam ^{[7
]}

Ebner, Florian ^{[7
,8
]}

Vapalahti, Olli ^{[9
,10
,11
]}

Otto, Markus ^{[12
]}

Bartenschlager, Ralf ^{[4
,13
]}

Herbeuval, Jean-Philippe ^{[14
]}

Schmidt-Chanasit, Jonas ^{[15
,16
]}

Roan, Nadia R. ^{[5
,6
]}

Muench, Jan ^{[1
,17
]}

机构：

[1] Ulm Univ, Inst Mol Virol, Med Ctr, D-89081 Ulm, Germany

[2] Ulm Univ, Inst Virol, Med Ctr, D-89081 Ulm, Germany

[3] Ulm Univ, Inst Epidemiol & Med Biometry, D-89075 Ulm, Germany

[4] Heidelberg Univ, Fac Med, Dept Infect Dis, Mol Virol, D-69120 Heidelberg, Germany

[5] Gladstone Inst Virol & Immunol, San Francisco, CA 94158 USA

[6] Univ Calif San Francisco, Dept Urol, San Francisco, CA 94158 USA

[7] Ulm Univ, Klin Frauenheilkunde & Geburtshilfe, Med Ctr, D-89081 Ulm, Germany

[8] Helios Amper Klin, Frauenklin, D-85221 Dachau, Germany

[9] Univ Helsinki, Dept Virol & Immunol, FIN-00014 Helsinki, Finland

[10] Helsinki Univ Hosp, Helsinki 00014, Finland

[11] Univ Helsinki, Dept Vet Biosci, FIN-00014 Helsinki, Finland

[12] Ulm Univ, Dept Neurol, D-89081 Ulm, Germany

[13] Heidelberg Univ, German Ctr Infect Res DZIF, Heidelberg Partner Site, D-69120 Heidelberg, Germany

[14] Univ Paris 05, Lab Chim & Biochim Pharmacol & Toxicol, Chem Biol Modeling & Immunotherapy CBMIT, CNRS,UMR8601,CICB Paris, F-75006 Paris, France

[15] World Hlth Org Collaborating Ctr Arbovirus & Hemo, Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

[16] German Ctr Infect Res DZIF, Partner Site Hamburg Luebeck Borstel, D-20359 Hamburg, Germany

[17] Ulm Univ, Med Ctr, Core Facil Funct Peptid, D-89081 Ulm, Germany

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

WEST NILE VIRUS; SEXUAL TRANSMISSION; SEMINAL PLASMA; AMYLOID FIBRILS; PREVENTION; DIAGNOSIS; OUTBREAK; BIOLOGY; BORNE;

D O I：

10.1038/s41467-018-04442-y

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zika virus (ZIKV) causes severe birth defects and can be transmitted via sexual intercourse. Semen from ZIKV-infected individuals contains high viral loads and may therefore serve as an important vector for virus transmission. Here we analyze the effect of semen on ZIKV infection of cells and tissues derived from the anogenital region. ZIKV replicates in all analyzed cell lines, primary cells, and endometrial or vaginal tissues. However, in the presence of semen, infection by ZIKV and other flaviviruses is potently inhibited. We show that semen prevents ZIKV attachment to target cells, and that an extracellular vesicle preparation from semen is responsible for this anti-ZIKV activity. Our findings suggest that ZIKV transmission is limited by semen. As such, semen appears to serve as a protector against sexual ZIKV transmission, despite the availability of highly susceptible cells in the anogenital tract and high viral loads in this bodily fluid.

引用

页数：14

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