The genomic organization of the open reading frame of the red pigment concentrating hormone gene in the blue crab Callinectes sapidus

被引：6

作者：

Martínez-Pérez, F

Valdés, J

Zinker, S

Aréchiga, H

机构：

[1] Univ Nacl Autonoma Mexico, Fac Med, Div Estudios Posgrado & Invest, Mexico City, DF, Mexico

[2] IPN, Ctr Invest & Estudios Avanzados, Dept Mol Biol & Genet, Mexico City, DF, Mexico

[3] IPN, Ctr Invest & Estudios Avanzados, Dept Bioquim, Mexico City, DF, Mexico

来源：

PEPTIDES | 2002年 / 23卷 / 04期

关键词：

crab; crustacean; gene; intron; invertebrate; neurohormone; neuropeptide; peptide; red pigment concentrating hormone;

D O I：

10.1016/S0196-9781(01)00648-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The open reading frame (ORF) of the gene for the precursor of the octapeptide Red Pigment Concentrating Hormone (RPCH) from the blue crab Callinectes sapidus was cloned by PCR with oligonucleotides targeted to the initiation and the end of the translation coding sequences. A 272 bp intron was characterized between nucleotides 343 and 344 of the reported cDNA, present in the region coding for the last amino acids of the precursor related peptide of RPCH. The intron genomic structure here described is similar to that reported for the gene coding for the Adipokinetic Hormone (AKH) of the grasshopper Schistocerea nitans. (C) 2002 Elsevier Science Inc. All rights reserved.

引用

页码：781 / 786

页数：6

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