Investigation on Sulfamethazine Molecularly Imprinted Two-dimensional Photonic Crystal Hydrogel Sensor

A novel sensor for the rapid and label-free detection of sulfamethazine, based on the combination of photonic crystal and molecular imprinting technique, was developed. The molecularly imprinted photonic hydrogel (MIPH) was prepared using sulfamethazine as the template molecule. The results showed that the diameter of Debye ring increased 0.9 cm when the concentration of SM2 changed from 0 to 1x10(-6) mol/L, namely, the lattice spacing decreased 46 nm. When the ratio of crosslinking of MIPH is 10% and the molar ratio of imprinting molecule to functional monomer is 1:75, the diameter of Debye ring is the largest. In addition, the diameter of Debye ring only increased 0.2, 0.25, 0.3 and 0.4 cm, respectively when the hydrogel immersed in 1x10(-6) mol/L roxithromycin (RM), tetracycline (TE), sulfonisoxazole (SIZ) and sulfadiazine (SDZ) solution, indicating that the MIPH had a high selectivity. The sensor was also tested for simulated water samples and sulfadimethanol veterinary tablets, and all of them had high responsiveness. The application of such a sensor with high selectivity, high sensitivity, and easy operation might offer a new method for rapid real-time detection of Sulfamethazine.