Detection of single nucleotide polymorphisms by PCR conformation-difference gel electrophoresis

被引：7
作者：
Zhu, Weifeng [1 ]
Deng, Yan [2 ]
Jie, Kemin [1 ]
Luo, Daya [1 ]
Liu, Zhuoqi [1 ]
Yu, Lehan [1 ]
Zeng, Erming [3 ]
Wan, Fusheng [1 ]
机构：
[1] Nanchang Univ, Coll Basic Med Sci, Dept Biochem & Mol Biol, Nanchang 330006, Peoples R China
[2] Nanchang Univ, Affiliated Hosp 2, Dept Pediat Ophthalmol, Nanchang 330006, Peoples R China
[3] Nanchang Univ, Affiliated Hosp 1, Dept Neurosurg, Nanchang 330006, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Conformation-sensitive gel electrophoresis; High throughput; PCR-conformation-difference gel electrophoresis; Single nucleotide polymorphisms; ACUTE LYMPHOBLASTIC-LEUKEMIA; C609T POLYMORPHISM; OXIDOREDUCTASE; RISK; SUSCEPTIBILITY; GENE; NQO1; SNP; DNA; ASSOCIATION;
D O I：
10.1007/s10529-012-1115-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The most common genetic variations in the human genome, single nucleotide polymorphisms (SNPs), are ideal biomarkers and are used extensively in disease research. Here we introduce a novel method of PCR-conformation-difference gel electrophoresis (PCR-CDGE) used for detecting SNPs. The principle of PCR-CDGE relies PCR products from different homozygous DNA samples showing dissimilar migration patterns upon PAGE due to their conformational differences. PCR products from heterozygous DNA samples may exhibit two or more bands in PAGE because of the existence of DNA homoduplexes and heteroduplexes. In this study, analysis of two SNPs showed that PCR-CDGE is an accurate, simple, rapid, low-cost, and high-throughput genotyping method that could be used in most laboratories.
引用
收藏
页码：515 / 522
页数：8
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