Number and spatial distribution of intrinsically photosensitive retinal ganglion cells in the adult albino rat

被引：66

作者：

Galindo-Romero, C. ^{[1
]}

Jimenez-Lopez, M. ^{[1
]}

Garcia-Ayuso, D. ^{[1
]}

Salinas-Navarro, M. ^{[1
]}

Nadal-Nicolas, P. M. ^{[1
,2
]}

Agudo-Barriuso, M. ^{[1
,2
]}

Villegas-Perez, M. P. ^{[1
]}

Aviles-Trigueros, M. ^{[1
]}

Vidal-Sanz, M. ^{[1
]}

机构：

[1] Univ Murcia, Dept Oftalmol, Fac Med, Inst Murciano Invest Biosanitarias, E-30100 Murcia, Spain

[2] Hosp Univ Virgen Arrixaca, Unidad Invest, Fdn Formac & Invest Sanitarias Reg Murcia, Inst Murciano Invest Biosanitarias, Murcia, Spain

来源：

EXPERIMENTAL EYE RESEARCH | 2013年 / 108卷

关键词：

melanopsin; Brn3a; retrograde axonal tracing; neighbour map; automated quantification; retinal ganglion cells; adult albino rat; TRANSCRIPTION FACTORS BRN3A; PUPILLARY LIGHT REFLEX; COMPUTERIZED ANALYSIS; MAMMALIAN RETINA; KNOCKOUT MICE; BLIND MICE; MELANOPSIN; MOUSE; PHOTORECEPTORS; POPULATION;

D O I：

10.1016/j.exer.2012.12.010

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Intrinsically photosensitive retinal ganglion cells (ipRGCs) respond directly to light and are responsible of the synchronization of the circadian rhythm with the photic stimulus and for the pupillary light reflex. To quantify the total population of rat-ipRGCs and to assess their spatial distribution we have developed an automated routine and used neighbour maps. Moreover, in all analysed retinas we have studied the general population of RGCs - identified by their Brn3a expression - and the population of ipRGCs - identified by melanopsin immunodetection - thus allowing the co-analysis of their topography. Our results show that the total mean number +/- standard deviation of ipRGCs in the albino rat is 2047 +/- 309. Their distribution in the retina seems to be complementary to that of Brn3a(+)RGCs, being denser in the periphery, especially in the superior retina where their highest densities are found in the temporal quadrant, above the visual streak. In addition, by tracing the retinas from both superior colliculi, we have also determined that 90.62% of the ipRGC project to these central targets. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：84 / 93

页数：10

共 55 条

[11] Axotomy-induced retinal ganglion cell death in adult mice: Quantitative and topographic time course analyses [J].