Establishment and validation of a method for multi-dose irradiation of cells in 96-well microplates

被引：15

作者：

Abatzoglou, Ioannis ^{[1
]}

Zois, Christos E. ^{[1
]}

Pouliliou, Stamatia ^{[1
]}

Koukourakis, Michael I. ^{[1
]}

机构：

[1] Democritus Univ Thrace, Dept Radiotherapy Oncol, Radiobiol & Radiopathol Unit, Alexandroupolis 68100, Greece

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 431卷 / 03期

关键词：

96-Well plate; Microplate; Radiation; Dose-response;

D O I：

10.1016/j.bbrc.2012.12.146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microplates are useful tools in chemistry, biotechnology and molecular biology. In radiobiology research, these can be also applied to assess the effect of a certain radiation dose delivered to the whole microplate, to test radio-sensitivity, radio-sensitization or radio-protection. Whether different radiation doses can be accurately applied to a single 96-well plate to further facilitate and accelerated research by one hand and spare funds on the other, is a question dealt in the current paper. Following repeated ion-chamber, TLD and radiotherapy planning dosimetry we established a method for multi-dose irradiation of cell cultures within a 96-well plate, which allows an accurate delivery of desired doses in sequential columns of the microplate. Up to eight different dose levels can be tested in one microplate. This method results in fast and reliable estimation of radiation dose-response curves. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：456 / 459

页数：4