Objective: To examine perceived poor sleep quality and its association with unhealthy lifestyle and self-reported morbidities among Brazilian workers. Methods: This study was part of a national survey involving 47,477 workers who received a questionnaire about lifestyle. Chi-square tests and logistic regression (OR = Odds Ratio) were conducted. Results: Poor sleep quality was reported by 21% of workers and was more prevalent among women (p < 0.01). After being adjusted for socio-demographic indicators, negative perception of health, wellbeing, stress, self-reported morbidities, physical inactivity during leisure-time (OR: 1.18) in both genders, and inadequate fruit and vegetable intake (ORmen: 1.15; ORwomen: 1.17), as well as excessive alcohol consumption (OR: 1.15) in men remained associated with poor sleep quality. With respect to morbidities, only diabetes in women (OR: 1.46) and hypertension in men (OR: 1.18) were associated with poor sleep quality. Conclusions: Our findings indicate that certain unhealthy behaviors and self-reported morbidities were associated with poor sleep quality. Efficient initiatives should be implemented to promote a healthier lifestyle among workers, which could reduce or control glucose levels and blood pressure in addition to improving perceived sleep quality. (C) 2012 Elsevier B.V. All rights reserved.