In Vitro Osteogenic/Dentinogenic Potential of an Experimental Calcium Aluminosilicate Cement

被引：31

作者：

Eid, Ashraf A. ^{[1
]}

Niu, Li-na ^{[2
]}

Primus, Carolyn M. ^{[3
]}

Opperman, Lynne A. ^{[4
]}

Pashley, David H. ^{[5
]}

Watanabe, Ikuya ^{[1
]}

Tay, Franklin R. ^{[5
,6
]}

机构：

[1] Nagasaki Univ, Dept Dent & Biomed Mat Sci, Grad Sch Biomed Sci, Nagasaki 852, Japan

[2] Fourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, Xian 710032, Peoples R China

[3] Primus Consulting, Bradenton, FL USA

[4] Texas A&M Univ, Baylor Coll Dent, Ctr Craniofacial Res & Diag, Dept Biomed Sci, Dallas, TX USA

[5] Georgia Hlth Sci Univ, Dept Oral Biol, Augusta, GA 30912 USA

[6] Georgia Hlth Sci Univ, Dept Endodont, Augusta, GA 30912 USA

来源：

JOURNAL OF ENDODONTICS | 2013年 / 39卷 / 09期

关键词：

Alizarin red S staining; alkaline phosphatase; calcium aluminosilicate cement; MDPC-23; cells; quantitative reverse transcription polymerase chain reaction; MINERAL TRIOXIDE AGGREGATE; DENTIN SIALOPHOSPHOPROTEIN; ALUMINATE CEMENT; MATRIX PROTEINS; ACID; DIFFERENTIATION; SIALOPROTEIN; ABILITY; REPAIR; BONE;

D O I：

10.1016/j.joen.2013.04.005

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Introduction: Calcium aluminosilicate cements are fast-setting, acid-resistant, bioactive cements that may be used as root-repair materials. This study examined the osteogenic/dentinogenic potential of an experimental calcium aluminosilicate cement (Quick-Set) by using a murine odontoblast-like cell model. Methods: Quick-Set and white ProRoot MTA (WMTA) were mixed with the proprietary gel or deionized water, allowed to set completely in 100% relative humidity, and aged in complete growth medium for 2 weeks until rendered non-cytotoxic. Similarly aged Teflon disks were used as negative control. The MDPC-23 cell line was used for evaluating changes in mRNA expressions of genes associated with osteogenic/dentinogenic differentiation and mineralization (quantitative reverse transcription polymerase chain reaction), alkaline phosphatase enzyme production, and extracellular matrix mineralization (alizarin red S staining). Results: After MDPC-23 cells were incubated with the materials in osteogenic differentiation medium for 1 week, both cements showed up-regulation in ALP and DSPP expression. Fold increases in these 2 genes were not significantly different between Quick-Set and WMTA. Both cements showed no statistically significant up-regulation/down-regulation in RUNX2, OCN, BSP, and DMP1 gene expression compared with Teflon. Alkaline phosphatase activity of cells cultured on Quick-Set and WMTA were not significantly different at 1 week or 2 weeks but were significantly higher (P < .05) than Teflon in both weeks. Both cements showed significantly higher calcium deposition compared with Teflon after 3 weeks of incubation in mineralizing medium (P < .001). Differences between Quick-Set and WMTA were not statistically significant. Conclusions: The experimental calcium aluminosilicate cement exhibits similar osteogenic/dentinogenic properties to WMTA and may be a potential substitute for commercially available tricalcium silicate cements.

引用

页码：1161 / 1166

页数：6

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