Plasma citrulline measurement using UPLC tandem mass-spectrometry to determine small intestinal enterocyte pathology

被引:58
作者
Demacker, Pierre N. M. [1 ]
Beijers, Antonius M. [1 ]
van Daal, Henny [1 ]
Donnelly, J. Peter [2 ]
Blijlevens, Nicole M. A. [2 ]
van den Ouweland, Johannes M. W. [1 ]
机构
[1] Canisius Wilhelmina Hosp, Dept Clin Chem, NL-6532 SZ Nijmegen, Netherlands
[2] Radboud Univ Nijmegen Med Ctr, Dept Hematol, Nijmegen, Netherlands
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2009年 / 877卷 / 04期
关键词
Citrulline; UPLC; Mass spectrometry; Mucosal barrier injury; Amino acid marker; HILIC chromatography; PERFORMANCE LIQUID-CHROMATOGRAPHY; AMINO-ACID-ANALYSIS; MARKER; EXCHANGE; DAMAGE; URINE;
D O I
10.1016/j.jchromb.2008.12.041
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Citrulline is a nonessential free amino acid, detectable in various biological fluids such as plasma, urine and cerebrospinal fluid. The plasma citrulline concentration is increasingly considered to be a reliable biomarker of enterocyte function. Current analysis usually involves lengthy HPLC separations as a part of classical amino acid profiling, or mass spectrometry usually in combination with derivatization. We employed UPLC-HILIC-tandem mass-spectrometry (MS/MS) of acetonitrile-derived supernatants from plasma samples of control subjects and of patients who had received myeloablative chemotherapy. Detection was achieved by the selected reaction monitoring of transitions: m/z 176 -> 70 and 180 -> 74 (for the deuterated standard), respectively. The method was precise and accurate with inter-day CV < 3.9% (n = 30), recoveries ranging from 98.0 to 100.3% and high linearity from 0.3 to at least 2000 mu Lmol/L. The results for 202 plasma samples agreed well with those obtained by the classical HPLC-fluorescence method. By a simple protein precipitation/extraction step and the UPLC separation the result can be available within 30 min of receipt with a capacity of at least 12 assays per hour. Citrulline in blood and plasma or serum was stable for at least 2 days at room temperature which would permit postal transport to the laboratory. The UPLC-MS/MS method for measuring plasma citrulline concentrations is fast and robust and is therefore an ideal tool for monitoring the intestinal enterocyte capacity of patients with various pathological conditions. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:387 / 392
页数:6
相关论文
共 25 条
  • [1] New biological indicators to evaluate and monitor radiation-induced damage: An accident case report
    Bertho, J. M.
    Roy, L.
    Souidi, M.
    Benderitter, M.
    Gueguen, Y.
    Lataillade, J. J.
    Prat, M.
    Fagot, T.
    De Revell, T.
    Gourmelon, P.
    [J]. RADIATION RESEARCH, 2008, 169 (05) : 543 - 550
  • [2] Citrulline: a potentially simple quantitative marker of intestinal epithelial damage following myeloablative therapy
    Blijlevens, NMA
    Lutgens, LCHW
    Schattenberg, AVMB
    Donnelly, JP
    [J]. BONE MARROW TRANSPLANTATION, 2004, 34 (03) : 193 - 196
  • [3] Chuang CK, 1998, CLIN CHEM, V44, P1052
  • [4] Plasma citrulline: A marker of enterocyte mass in villous atrophy-associated small bowel disease
    Crenn, P
    Vahedi, K
    Lavergne-Slove, A
    Cynober, L
    Matuchansky, C
    Messing, B
    [J]. GASTROENTEROLOGY, 2003, 124 (05) : 1210 - 1219
  • [5] Citrulline as a biomarker of intestinal failure due to enterocyte mass reduction
    Crenn, Pascal
    Messing, Bernard
    Cynober, Luc
    [J]. CLINICAL NUTRITION, 2008, 27 (03) : 328 - 339
  • [6] Almost all about citrulline in mammals
    Curis, E
    Nicolis, I
    Moinard, C
    Osowska, S
    Zerrouk, N
    Bénazeth, S
    Cynober, L
    [J]. AMINO ACIDS, 2005, 29 (03) : 177 - 205
  • [7] Rapid comprehensive amino acid analysis by liquid chromatography/tandem mass spectrometry: comparison to cation exchange with post-column ninhydrin detection
    Dietzen, Dennis J.
    Weindel, Annette L.
    Carayannopoulos, Mary O.
    Landt, Michael
    Normansell, Ellen T.
    Reimschisel, Tyler E.
    Smith, Carl H.
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (22) : 3481 - 3488
  • [8] Bacteraemia coincides with low citrulline concentrations after high-dose melphalan in autologous HSCT recipients
    Herbers, A. H. E.
    Blijlevens, N. M. A.
    Donnelly, J. P.
    de Witte, T. J. M.
    [J]. BONE MARROW TRANSPLANTATION, 2008, 42 (05) : 345 - 349
  • [9] Age-related serial plasma citrulline levels in preterm neonates
    Ioannou, Hariklia
    Avgoustides-Savvopoulou, Persephone
    Diamanti, Elisavet
    Tsampoura, Zoi
    Drosou-Agakidou, Vasiliki
    [J]. PEDIATRICS, 2008, 121 : S137 - S138
  • [10] ORTHO-PHTHALDIALDEHYDE PRECOLUMN DERIVATIZATION AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF POLYPEPTIDE HYDROLYSATES AND PHYSIOLOGICAL FLUIDS
    JONES, BN
    GILLIGAN, JP
    [J]. JOURNAL OF CHROMATOGRAPHY, 1983, 266 (AUG): : 471 - 482