Isolation and characterization of eighteen polymorphic microsatellite loci for the topical seagrass, Syringodium isoetifolium

被引：5

作者：

Wainwright, Benjamin J. ^{[1
]}

Arlyza, Irma S. ^{[2
]}

Karl, Stephen A. ^{[1
]}

机构：

[1] Univ Hawaii Manoa, Hawaii Inst Marine Biol, Kaneohe, HI 96744 USA

[2] Indonesian Inst Sci, Res Ctr Oceanog, Jakarta 14430, Indonesia

来源：

CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 04期

关键词：

Connectivity; Gene flow; Indonesia; Conservation; ABUNDANCE; PRIMERS;

D O I：

10.1007/s12686-013-9938-0

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Eighteen polymorphic microsatellite loci were developed for the tropical seagrass, Syringodium isoetifolium. Microsatellite enriched genomic libraries were constructed and subsequently sequenced using Pacific Biosciences SMRT technology. The 18 loci were characterized in 24 individuals from the Indonesian archipelago. Observed levels of heterozygosity ranged from 0.14 to 0.91 with a mean of 3.7 alleles per locus. Four pairs of loci showed evidence of significant linkage disequilibrium and one locus deviated significantly from Hardy-Weinberg expectations.

引用

页码：943 / 945

页数：3

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