A novel PCR-based genotyping scheme for clinical Klebsiella pneumoniae

被引：27
|
作者：
Chen, Zhenhong [1 ]
Liu, Mengying [2 ,3 ]
Cui, Yujun [2 ]
Wang, Li [2 ,3 ]
Zhang, Yiquan [2 ]
Qiu, Jingfu [3 ]
Yang, Ruifu [2 ,4 ]
Liu, Changting [1 ]
Zhou, Dongsheng [2 ]
机构：
[1] Chinese Peoples Liberat Army Gen Hosp, Nanlou Resp Dis Dept, Beijing 100853, Peoples R China
[2] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
[3] Chongqing Med Univ, Sch Publ Hlth, Chongqing 400016, Peoples R China
[4] Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China
来源：
FUTURE MICROBIOLOGY | 2014年 / 9卷 / 01期
基金：
中国国家自然科学基金;
关键词：
genotyping; Klebsiella pneumoniae; large variably presented gene cluster; serotype marker; virulence loci; PYOGENIC LIVER-ABSCESS; CAPSULAR POLYSACCHARIDE SYNTHESIS; COMPLETE GENOME SEQUENCE; VIRULENCE; GENE; EVOLUTION; INFECTION; LIPOPOLYSACCHARIDE; IDENTIFICATION; COLONIZATION;
D O I：
10.2217/fmb.13.137
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To establish a PCR-based genotyping method for clinical Klebsiella pneumoniae. Materials & methods: The prevalence of six serotype markers, 41 large variably presented gene clusters, and seven additional virulence markers were screened by PCR in 327 clinical K. pneumoniae strains from China. Results: Detection of serotype markers enabled the identification of capsular serotypes K1, K2, K5, K20, K54 and K57. K. pneumoniae isolates of different origins gave distinct profiles of virulence loci, allowing us to gain a full overview of virulence gene distribution of the strains tested. A novel genotyping scheme was established to group clinical K. pneumoniae strains into distinct complexes based on the profiles of large variably presented gene clusters and virulence markers. Conclusion: This PCR-based genotyping method would be useful to not only characterize genetic diversity and virulence gene distribution, but also for genotyping, origin tracing and risk estimation of K. pneumoniae.
引用
收藏
页码：21 / 32
页数：12
相关论文
共 50 条
  • [21] Comparison of three PCR-based assays for SNP genotyping in plants
    Chiara Broccanello
    Claudia Chiodi
    Andrew Funk
    J. Mitchell McGrath
    Lee Panella
    Piergiorgio Stevanato
    Plant Methods, 14
  • [22] Genotyping and Virulence Analysis of Drug Resistant Clinical Klebsiella pneumoniae Isolates in Egypt
    Abdelhamid, Sarah M.
    Abd-Elaal, Hala Mohamed
    Matareed, Moustafa Osama
    Baraka, Kholoud
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2020, 14 (03): : 1967 - 1975
  • [23] Bioinformatic Tools for Genotyping of Klebsiella pneumoniae Isolates
    Nykrynova, Marketa
    Maderankova, Denisa
    Bezdicek, Matej
    Lengerova, Martina
    Skutkova, Helena
    INFORMATION TECHNOLOGY IN BIOMEDICINE (ITIB 2018), 2019, 762 : 419 - 428
  • [24] Comparison of Three PCR-Based Methods for the Detection of Mycoplasma pneumoniae (Mp) in Varied Clinical Specimens
    Lima, Jose
    Xiao, Li
    Ratliff, Amy
    Duffy, Lynn
    Waites, Ken B.
    AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 2009, 132 (03) : 470 - 470
  • [25] Evaluation of PCR-based approach for serotype determination of Streptococcus pneumoniae
    Shakrin, N. N. S. M.
    Balasubramaniam, S. D.
    Yusof, H. A.
    Mastuki, M. F.
    Masri, S. N.
    Taib, N. M.
    Nordin, S. A.
    Jamal, F.
    Clarke, S. C.
    Desa, M. N. M.
    TROPICAL BIOMEDICINE, 2013, 30 (02) : 338 - 344
  • [26] A one-step multiplex PCR to identify Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae in the clinical routine
    Fonseca, Erica Lourenco
    Ramos, Nilceia da Veiga
    Nascimento Andrade, Bruno G.
    Morais, Lena L. C. S.
    Abanto Marin, Michel F.
    Vicente, Ana Carolina P.
    DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2017, 87 (04) : 315 - 317
  • [27] Application of a PCR-Based Cytoplasm Genotyping Method for Phylogenetic Analysis in Potato
    Kazuyoshi Hosaka
    Rena Sanetomo
    American Journal of Potato Research, 2014, 91 : 246 - 253
  • [28] Specificity and sensitivity of RHD genotyping methods by PCR-based DNA amplification
    Aubin, JT
    Kim, CL
    Mouro, I
    Colin, Y
    Bignozzi, C
    Brossard, Y
    Cartron, JP
    BRITISH JOURNAL OF HAEMATOLOGY, 1997, 98 (02) : 356 - 364
  • [29] Pitfalls of PCR-based strategy for genotyping cre-loxP mice
    Zhang, Jihui
    Liu, Wen
    Ye, Ping
    D'Ercole, A. Joseph
    BIOTECHNIQUES, 2007, 42 (03) : 281 - +
  • [30] Development of a PCR-based assay for detection, quantification, and genotyping of human adenoviruses
    Chmielewicz, B
    Nitsche, A
    Schweiger, B
    Ellerbrok, H
    CLINICAL CHEMISTRY, 2005, 51 (08) : 1365 - 1373
12345