Bone marrow mesenchymal stem cells promote head and neck cancer progression through Periostin-mediated phosphoinositide 3-kinase/Akt/mammalian target of rapamycin

被引:55
作者
Liu, Chuanxia [1 ]
Feng, Xiaoxia [1 ]
Wang, Baixiang [1 ]
Wang, Xinhua [1 ]
Wang, Chaowei [1 ]
Yu, Mengfei [1 ]
Cao, Guifen [1 ]
Wang, Huiming [1 ]
机构
[1] Zhejiang Univ, Coll Med, Affiliated Hosp Stomatol, Hangzhou, Zhejiang, Peoples R China
关键词
bone marrow mesenchymal stem cells; epithelial mesenchymal transition; head and neck cancer; Periostin; PI3K; Akt; mTOR; TUMOR MICROENVIRONMENT; BREAST-CANCER; CARCINOMA; GROWTH; METASTASIS; EXPRESSION; STROMA;
D O I
10.1111/cas.13479
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Bone marrow mesenchymal stem cells (BMMSC) have been shown to be recruited to the tumor microenvironment and exert a tumor-promoting effect in a variety of cancers. However, the molecular mechanisms related to the tumor-promoting effect of BMMSC on head and neck cancer (HNC) are not clear. In this study, we investigated Periostin (POSTN) and its roles in the tumor-promoting effect of BMMSC on HNC. In vitro analysis of HNC cells cultured in BMMSC-conditioned media (MSC-CM) showed that MSC-CM significantly promoted cancer progression by enhancing cell proliferation, migration, epithelial-mesenchymal transformation (EMT), and altering expression of cell cycle regulatory proteins and inhibition of apoptosis. Moreover, MSC-CM promoted the expression of POSTN and POSTN promoted HNC progression through the activation of the phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway. In a murine model of HNC, we found that BMMSC promoted tumor growth, invasion, metastasis and enhanced the expression of POSTN and EMT in tumor tissues. Clinical sample analysis further confirmed that the expression of POSTN and N-cadherin were correlated with pathological grade and lymph node metastasis of HNC. In conclusion, this study indicated that BMMSC promoted proliferation, invasion, survival, tumorigenicity and migration of head and neck cancer through POSTN-mediated PI3K/Akt/mTOR activation.
引用
收藏
页码:688 / 698
页数:11
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