FRET-based colorimetric and ratiometric sensor for visualizing pH change and application for bioimaging in living cells, bacteria and zebrafish

被引：28

作者：

He, Xiaojun ^{[1
]}

Ding, Feng ^{[4
]}

Xu, Wei ^{[1
]}

Xu, Chuchu ^{[1
]}

Li, Yahui ^{[1
]}

Qian, Yuna ^{[2
]}

Zhao, Shengzhe ^{[2
]}

Chen, Hong ^{[3
]}

Shen, Jianliang ^{[1
,2
]}

机构：

[1] Wenzhou Med Univ, Sch Biomed Engn, Sch Ophthalmol & Optometry, Wenzhou 325035, Zhejiang, Peoples R China

[2] Univ Chinese Acad Sci, Engn Res Ctr Clin Funct Mat & Diag & Treatment De, Wenzhou 325001, Peoples R China

[3] Luoyang Normal Univ, Coll Food & Drug, Luoyang Key Lab Organ Funct Mol, Luoyang 471934, Peoples R China

[4] Wenzhou Med Univ, Sch Basic Med Sci, Dept Microbiol & Immunol, Wenzhou 325035, Zhejiang, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2020年 / 1127卷

基金：

中国国家自然科学基金;

关键词：

pH sensor; Colorimetric and ratiometric; FRET; Bioimaging; INFRARED FLUORESCENT-PROBES; NEUTRAL PH; IN-VITRO; DESIGN; LEVEL; CU2+; HG2+;

D O I：

10.1016/j.aca.2020.06.031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Acid-alkaline balance plays a crucial role in all biological processes. Accordingly, monitoring pH changes will help us to understand the functional status of these physiological and pathological processes. Though fluorescent probes may be a useful tool for detecting pH changes, and there are many limitations to currently available probes, such as background interference, potential cytotoxicity, and poor cell permeability, which call for a solution urgently. In this work, a rhodamine-derived colorimetric and ratiometric sensor (Rh-HN) was fabricated for monitoring pH change via the mechanism of fluorescence resonance energy transfer (FRET). Rh-HN has been shown to possess several advantages over other probes, such as high sensitivity, outstanding permeability, and low toxicity. Besides, the fluorescence intensity ratio (F-526/F-592) of Rh-HN displays a pH-sensitive response from 2.0 to 7.5 (pKa = 5.05) and linear response from pH 3.8 to 6.4, which was desirable for mapping pH change in the biological systems. Besides, the results indicated that Rh-HN generated a pH-dependent response regulated by switchable forms between closed and opened spirolactam ring. Overall, Rh-HN has accomplished sensing and mapping of pH in living cells, bacteria, and zebrafish. Those results demonstrated that the great potential of Rh-HN in sensing and visualizing pH in the living biosystem. (C) 2020 Elsevier B.V. All rights reserved.

引用

页码：29 / 38

页数：10

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