The PDT activity of free and pegylated pheophorbide a against an amelanotic melanoma transplanted in C57/BL6 mice

被引：24

作者：

Rapozzi, Valentina ^{[1
]}

Zorzet, Sonia ^{[2
]}

Zacchigna, Marina ^{[3
]}

Drioli, Sara ^{[3
]}

Xodo, Luigi E. ^{[1
]}

机构：

[1] Sch Med, Dept Med & Biol Sci, I-33100 Udine, Italy

[2] Univ Trieste, Dept Life Sci, I-34100 Trieste, Italy

[3] Univ Trieste, Dept Chem & Pharmaceut Sci, I-34100 Trieste, Italy

来源：

INVESTIGATIONAL NEW DRUGS | 2013年 / 31卷 / 01期

关键词：

Pheophorbide a; mPEG conjugation; Photodynamic therapy; Melanoma; Mice; IN-VIVO EFFICACY; PHOTODYNAMIC THERAPY; TARGETED DELIVERY; TUMOR-CELLS; PHOTOSENSITIZER; CANCER; CHLOROPHYLL; NANOPARTICLES; KINASE; VITRO;

D O I：

10.1007/s10637-012-9844-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Pheophorbide a (Pba) is a chlorophyll catabolite that has been proposed as photosensitizer in photodynamic therapy. In a previous study we conjugated Pba to monomethoxy-polyethylene glycol (mPEG-Pba), to increase its solubility and pharmacokinetics. Here, we compare the photodynamic therapy efficacy of free Pba and mPEG-Pba to cure a subcutaneous amelanotic melanoma transplanted in C57/BL6 mice. The photosensitizers, i.p. injected (30 mg/kg), showed no toxicity when the animals were kept in the dark. But, after photoactivation with a 660 nm laser (fluence of 193 J/cm(2)), both photosensitizers, in particular mPEG-Pba, showed a strong efficacy to cure the tumor, both in terms of tumor growth delay and increase of Kaplan-Meier median survival time. Together, our in vivo data demonstrate that mPEG-conjugated Pba is a promising photosensitizer for the photodynamic therapy of cancer.

引用

页码：192 / 199

页数：8

共 53 条

[1] Epidermal growth factor receptor-targeted photosensitizer selectively inhibits EGFR signaling and induces targeted phototoxicity in ovarian cancer cells [J].

Abu-Yousif, Adnan O. ;

Moor, Anne C. E. ;

Zheng, Xiang ;

Savellano, Mark D. ;

Yu, Weiping ;

Selbo, Pal K. ;

Hasan, Tayyaba .

CANCER LETTERS, 2012, 321 (02) :120-127

[2] Photodynamic Therapy of Cancer: An Update [J].

Agostinis, Patrizia ;

Berg, Kristian ;

Cengel, Keith A. ;

Foster, Thomas H. ;

Girotti, Albert W. ;

Gollnick, Sandra O. ;

Hahn, Stephen M. ;

Hamblin, Michael R. ;

Juzeniene, Asta ;

Kessel, David ;

Korbelik, Mladen ;

Moan, Johan ;

Mroz, Pawel ;

Nowis, Dominika ;

Piette, Jacques ;

Wilson, Brian C. ;

Golab, Jakub .

CA-A CANCER JOURNAL FOR CLINICIANS, 2011, 61 (04) :250-281

[3] Protein oxidation in aging, disease, and oxidative stress [J].

Berlett, BS ;

Stadtman, ER .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (33) :20313-20316

[4] Targeted photodynamic therapy with multiply-loaded recombinant antibody fragments [J].

Bhatti, Manpreet ;

Yahioglu, Gokhan ;

Milgrom, Lionel R. ;

Garcia-Maya, Mitla ;

Chester, Kerry A. ;

Deonarain, Mahendra P. .

INTERNATIONAL JOURNAL OF CANCER, 2008, 122 (05) :1155-1163

[5] The in vivo efficacy of phthalocyanine-nanoparticle conjugates for the photodynamic therapy of amelanotic melanoma [J].

Camerin, Monica ;

Magaraggia, Michela ;

Soncin, Marina ;

Jori, Giulio ;

Moreno, Miguel ;

Chambrier, Isabelle ;

Cook, Michael J. ;

Russell, David A. .

EUROPEAN JOURNAL OF CANCER, 2010, 46 (10) :1910-1918

[6]

Campaner P., 2006, LETT ORG CHEM, V10, P773

[7] Photodynamic Treatment Induces an Apoptotic Pathway Involving Calcium, Nitric Oxide, p53, p21-Activated Kinase 2, and c-Jun N-Terminal Kinase and Inactivates Survival Signal in Human Umbilical Vein Endothelial Cells [J].

Chan, Wen-Hsiung .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (02) :1041-1059

[8] Non Polymeric Nanoparticles for Photodynamic Therapy Applications: Recent Developments [J].

Chouikrat, R. ;

Seve, A. ;

Vanderesse, R. ;

Benachour, H. ;

Barberi-Heyob, M. ;

Richeter, S. ;

Raehm, L. ;

Durand, J. -O. ;

Verelst, M. ;

Frochot, C. .

CURRENT MEDICINAL CHEMISTRY, 2012, 19 (06) :781-792

[9] Silica-based nanoparticles for photodynamic therapy applications [J].

Couleaud, Pierre ;

Morosini, Vincent ;

Frochot, Celine ;

Richeter, Sebastien ;

Raehm, Laurence ;

Durand, Jean-Olivier .

NANOSCALE, 2010, 2 (07) :1083-1095

[10] Transferrin-conjugated liposome targeting of photosensitizer AlPcS4 to rat bladder carcinoma cells [J].

Derycke, ASL ;

Kamuhabwa, A ;

Gijsens, A ;

Roskams, T ;

De Vos, D ;

Kasran, A ;

Huwyler, J ;

Missiaen, L ;

de Witte, PAM .

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2004, 96 (21) :1620-1630

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