Protective effect of N-acetylcysteine on fenitrothion-induced toxicity: The antioxidant status and metabolizing enzymes expression in rats

被引:28
作者
Galal, Azza A. A. [1 ]
Ramadan, Raghda A. [1 ]
Metwally, Mohamed M. M. [2 ]
El-Sheikh, Sawsan M. A. [1 ]
机构
[1] Zagazig Univ, Dept Pharmacol, Fac Vet Med, Zagazig 44511, Egypt
[2] Zagazig Univ, Dept Pathol, Fac Vet Med, Zagazig 44511, Egypt
关键词
N-acetylcysteine; Fenitrothion; Oxidative stress; Inflammation; Gene expression; INDUCED OXIDATIVE STRESS; ACETYL CYSTEINE; SUBCHRONIC EXPOSURE; GENE-EXPRESSION; INFLAMMATION; LIVER; MECHANISMS; INDUCTION;
D O I
10.1016/j.ecoenv.2019.01.004
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The existence of fenitrothion (FNT) in the soil, water, and food products has harmful effects on non-target organisms. Therefore, this study was conducted to evaluate the hepatotoxic, nephrotoxic and neurotoxic effects of FNT and the possible ameliorative effect of N-acetylcysteine (NAC), a precursor of intracellular GSH, on FNT-induced toxicity. For this purpose, thirty-two adult male albino rats were allocated into control group and groups treated with NAC (200 mg/kg), FNT (10 mg/kg) and FNT + NAC via gastric tube daily for 28 days. FNT intoxication significantly reduced food intake, water intake, body weight, and body weight gain and altered the expression of phase I and phase II xenobiotic-metabolizing enzymes-cytochrome P450 (CYP1A1) and glutathione S-transferase (GSTA4-4). In hepatic, renal and brain tissues, FNT induced oxidative stress, hepatopathy, nephropathy, and encephalopathy, and significantly increased pro-inflammatory cytokines. Furthermore, FNT exposure significantly elevated the level of hepatic and renal injury biomarkers and significantly inhibited the brain acetylcholinesterase activity. Co-administration of NAC with FNT modulated most of these altered biochemical, oxidative and inflammatory markers and restored the xenobiotic-metabolizing enzymes expression and histological structures. Our study indicated the involvement of oxidative damage, inflammation, and alteration of xenobiotic-metabolizing enzymes expression in FNT-induced toxicity and revealed that they were significantly improved by NAC co-treatment. These findings suggest that NAC administration might protect against FNT-induced toxicity in non-target organisms, including humans.
引用
收藏
页码:502 / 510
页数:9
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