Extraction and isolation of flavonoid glycosides from Flos Sophorae Immaturus using ultrasonic-assisted extraction followed by high-speed countercurrent chromatography

被引:48
作者
Xie, Zhisheng [1 ,2 ]
Sun, Yongjiang [3 ]
Lam, Shingchung [1 ,2 ]
Zhao, Mingqian [1 ,2 ]
Liang, Zhikun [1 ,2 ]
Yu, Xiaoxue [1 ,2 ]
Yang, Depo [1 ,2 ]
Xu, Xinjun [1 ,2 ]
机构
[1] Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510275, Guangdong, Peoples R China
[2] Guangdong Technol Res Ctr Adv Chinese Med, Lab Qual Control Tradit Chinese Med, Guangzhou, Guangdong, Peoples R China
[3] Biozen Pharmaceut, Qual Adm & Risk Control Ctr, Xuchang, Peoples R China
关键词
Flavonoids; Flos Sophorae Immaturus; Isolation; Response surface methodology; Ultrasonic-assisted extraction; RESPONSE-SURFACE METHODOLOGY; PREPARATIVE ISOLATION; CAPILLARY-ELECTROPHORESIS; RUTIN; SEPARATION; PURIFICATION; OPTIMIZATION; ANTIOXIDANT; QUERCETIN; ALKALOIDS;
D O I
10.1002/jssc.201301340
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method of ultrasonic-assisted extraction followed by high-speed countercurrent chromatography was established for the extraction and isolation of three flavonoid glycosides, i.e. rutin, narcissin, and nicotiflorin from Flos Sophorae Immaturus. The effects of ultrasonic-assisted extraction factors for the main flavonoid compound (rutin) from Flos Sophorae Immaturus were optimized using Box-Behnken design combined with response surface methodology. The optimum conditions were determined as ultrasonic power 83% (600 W), solvent-to-material ratio 56:1, methanol concentration 82% v/v, and extraction time 60 min. Three bioactive flavonol glucosides, rutin, narcissin, and nicotiflorin were isolated from Flos Sophorae Immaturus using high-speed countercurrent chromatography. The separation was performed with a two-phase solvent system containing ethyl acetate/n-butanol/methanol/water (4:0.9:0.2:5, v/v). Amounts of 87 mg of rutin, 10.8 mg of narcissin, and 1.8 mg of nicotiflorin were isolated from 302 mg of crude extract of Flos Sophorae Immaturus in a one-step separation within 160 min with purities of 99.3, 98.0, and 95.1%, respectively, as determined by HPLC with diode array detection. Their structures were characterized by UV, MS, and NMR spectroscopy. It was demonstrated that the established method was simple, fast, and convenient, which was feasible to extract and isolate active flavonoid glycosides from Flos Sophorae Immaturus.
引用
收藏
页码:957 / 965
页数:9
相关论文
共 37 条
[1]   Antioxidant compounds from Ebenus pinnata [J].
Abreu, Pedro M. ;
Braham, Hatem ;
Ben Jannet, Hichem ;
Mighri, Zine ;
Matthew, Susan .
FITOTERAPIA, 2007, 78 (01) :32-34
[2]   Preparative isolation and purification of alkannin/shikonin derivatives from natural products by high-speed counter-current chromatography [J].
Assimopoulou, Andreana N. ;
Sturm, Sonja ;
Stuppner, Hermann ;
Papageorgiou, Vassilios P. .
BIOMEDICAL CHROMATOGRAPHY, 2009, 23 (02) :182-198
[3]   Response surface methodology (RSM) as a tool for optimization in analytical chemistry [J].
Bezerra, Marcos Almeida ;
Santelli, Ricardo Erthal ;
Oliveira, Eliane Padua ;
Villar, Leonardo Silveira ;
Escaleira, Luciane Amlia .
TALANTA, 2008, 76 (05) :965-977
[4]   Ethyl acetate extract and its major constituent, isorhamnetin 3-O-rutinoside, from Nitraria retusa leaves, promote apoptosis of human myelogenous erythroleukaemia cells [J].
Boubaker, J. ;
Bhouri, W. ;
Ben Sghaier, M. ;
Ghedira, K. ;
Franca, M. G. Dijoux ;
Chekir-Ghedira, L. .
CELL PROLIFERATION, 2011, 44 (05) :453-461
[5]   Strategies of solvent system selection for the isolation of flavonoids by countercurrent chromatography [J].
Costa, Fernanda das Neves ;
Leitao, Gilda Guimaraes .
JOURNAL OF SEPARATION SCIENCE, 2010, 33 (03) :336-347
[6]   Preparative isolation and purification of seven main antioxidants from Eucommia ulmoides Oliv. (Du-zhong) leaves using HSCCC guided by DPPH-HPLC experiment [J].
Dai, Xingping ;
Huang, Qiong ;
Zhou, Boting ;
Gong, Zhicheng ;
Liu, Zhaoqian ;
Shi, Shuyun .
FOOD CHEMISTRY, 2013, 139 (1-4) :563-570
[7]   The selected flavonol glycoside derived from Sophorae Flos improves glucose uptake and inhibits adipocyte differentiation via activation AMPK in 3T3-L1 cells [J].
Do Thi Ha ;
Trinh Nam Trung ;
Tran Thi Phuong ;
Yim, Namhui ;
Chen, Quan Cheng ;
Bae, KiHwan .
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2010, 20 (20) :6076-6081
[8]   Optimized ultrasound-assisted extraction procedure for the analysis of opium alkaloids in papaver plants by cyclodextrin-modified capillary electrophoresis [J].
Fakhari, Ali Reza ;
Nojavan, Saeed ;
Ebrahimi, Samad Nejad ;
Evenhuis, Christopher John .
JOURNAL OF SEPARATION SCIENCE, 2010, 33 (14) :2153-2159
[9]   Separation of alkaloids from herbs using high-speed counter-current chromatography [J].
Fang, Lei ;
Liu, Yuqin ;
Yang, Bin ;
Wang, Xiao ;
Huang, Luqi .
JOURNAL OF SEPARATION SCIENCE, 2011, 34 (19) :2545-2558
[10]   Box-Behnken design: An alternative for the optimization of analytical methods [J].
Ferreira, S. L. C. ;
Bruns, R. E. ;
Ferreira, H. S. ;
Matos, G. D. ;
David, J. M. ;
Brandao, G. C. ;
da Silva, E. G. P. ;
Portugal, L. A. ;
dos Reis, P. S. ;
Souza, A. S. ;
dos Santos, W. N. L. .
ANALYTICA CHIMICA ACTA, 2007, 597 (02) :179-186