Improving enzymatic hydrolysis of industrial hemp (Cannabis sativa L.) by electron beam irradiation

被引:42
作者
Shin, Soo-Jeong [2 ]
Sung, Yong Joo [1 ]
机构
[1] KT&G Cent Res Inst, Taejon 305805, South Korea
[2] Chungbuk Natl Univ, Cheongju 361763, Chungbuk, South Korea
关键词
Pretreatment; Electron beam irradiation; Hemp; Enzymatic hydrolysis; DRIFTS;
D O I
10.1016/j.radphyschem.2008.05.047
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The electron beam irradiation was applied as a pretreatment of the enzymatic hydrolysis of hemp biomass with doses of 150, 300 and 450 kGy. The higher irradiation dose resulted in the more extraction with hot-water extraction or 1% sodium hydroxide solution extraction. The higher solubility of the treated sample was originated from the chains scission during irradiation, which was indirectly demonstrated by the increase of carbonyl groups as shown in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) spectra. The changes in the micro-structure of hemp resulted in the better response to enzymatic hydrolysis with commercial cellulases (Celluclast 1.5L and Novozym 342). The improvement in enzymatic hydrolysis by the irradiation was more evident in the hydrolysis of the xylan than in that of the cellulose. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1034 / 1038
页数:5
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