Detection of Epstein-Barr virus RNA and related antigens in non-neoplastic lymphoid lesions

被引：0

作者：

Tonoyama, Y ^{[1
]}

Teramoto, N ^{[1
]}

Sarker, AB ^{[1
]}

Yoshino, T ^{[1
]}

Hayashi, K ^{[1
]}

Takahashi, K ^{[1
]}

Akagi, T ^{[1
]}

机构：

[1] OKAYAMA UNIV, SCH MED, DEPT PATHOL, OKAYAMA 700, JAPAN

来源：

ACTA MEDICA OKAYAMA | 1996年 / 50卷 / 02期

关键词：

EBER-I; BCRF-I; LMP-I; ZEBRA; lymphoid lesion;

D O I：

暂无

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

To elucidate the latent state and reactivation of Epstein-Barr virus (EBV) in non-neoplastic lymphoid lesions, we investigated 144 nonneoplastic lymphoid lesions by in situ hybridization (ISH) to detect the expression of EBV-encoded small RNAs (EBER)-1 and BCRF-1 and by immunostaining for latent membrane protein (LMP)-1 and ZEBRA. ISH for EBER-1 detected EBER-1 1-positive cells (EPC) in 31 of the 144 examined lesions (22%). EPC were detected in 4 of 49 cases of nonspecific lymphoid hyperplasia, in 16 of 20 abscess-forming granulomatous lymphadenitis (AFGL), 5 of 25 Kikuchi's disease, and in 3 of 3 infectious mononucleosis. LMP-1 was expressed in 6 of 124 non-neoplastic lymphoid lesions (4.8%). LMP-1-positive cells were observed in 6 of the 31 EBER-1-positive cases (19%). EPC were detected significantly more frequently in LMP-1- and ZEBRA-positive specimens than in the LMP-1- and ZEBRA-negative specimens. BCRF-1 was expressed in 4 of 11 cases examined: 2 of 3 AFGL, 1 of 2 Kikuchi's disease, and in the 1 case of atypical lymphoid hyperplasia. This study suggests that Epstein-Barr virus is prevalent and can be reactivated in the lymph nodes effaced by destructive inflammation, such as AFGL. Such inflammation may provide a local milieu that is conducive for EBV to enter the lyric cycle.

引用

页码：89 / 96

页数：8