Large-scale transcriptional activity in chromosomes 21 and 22

被引：638

作者：

Kapranov, P

Cawley, SE

Drenkow, J

Bekiranov, S

Strausberg, RL

Fodor, SPA

Gingeras, TR ^{[1
]}

机构：

[1] Affymetrix, Santa Clara, CA 95051 USA

[2] NCI, Bethesda, MD 20892 USA

来源：

SCIENCE | 2002年 / 296卷 / 5569期

关键词：

D O I：

10.1126/science.1068597

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The sequences of the human chromosomes 21 and 22 indicate that there are approximately 770 well-characterized and predicted genes. In this study, empirically derived maps identifying active areas of RNA transcription on these chromosomes have been constructed with the use of cytosolic polyadenylated RNA obtained from 11 human cell lines. Oligonucleotide arrays containing probes spaced on average every 35 base pairs along these chromosomes were used. When compared with the sequence annotations available for these chromosomes, it is noted that as much as an order of magnitude more of the genomic sequence is transcribed than accounted for by the predicted and characterized exons.

引用

页码：916 / 919

页数：4

共 14 条

[1] CLONING A BALANCED TRANSLOCATION ASSOCIATED WITH DIGEORGE-SYNDROME AND IDENTIFICATION OF A DISRUPTED CANDIDATE GENE [J].

BUDARF, ML ;

COLLINS, J ;

GONG, WL ;

ROE, B ;

WANG, ZL ;

BAILEY, LC ;

SELLINGER, B ;

MICHAUD, D ;

DRISCOLL, DA ;

EMANUEL, BS .

NATURE GENETICS, 1995, 10 (03) :269-278

[2] The human transcriptome map:: Clustering of highly expressed genes in chromosomal domains [J].

Caron, H ;

van Schaik, B ;

van der Mee, M ;

Baas, F ;

Riggins, G ;

van Sluis, P ;

Hermus, MC ;

van Asperen, R ;

Boon, K ;

Voûte, PA ;

Heisterkamp, S ;

van Kampen, A ;

Versteeg, R .

SCIENCE, 2001, 291 (5507) :1289-+

[3] The DNA sequence of human chromosome 22 [J].

Dunham, I ;

Shimizu, N ;

Roe, BA ;

Chissoe, S ;

Dunham, I ;

Hunt, AR ;

Collins, JE ;

Bruskiewich, R ;

Beare, DM ;

Clamp, M ;

Smink, LJ ;

Ainscough, R ;

Almeida, JP ;

Babbage, A ;

Bagguley, C ;

Balley, J ;

Barlow, K ;

Bates, KN ;

Beasley, O ;

Bird, CP ;

Blakey, S ;

Bridgeman, AM ;

Buck, D ;

Burgess, J ;

Burrill, WD ;

Burton, J ;

Carder, C ;

Carter, NP ;

Chen, Y ;

Clark, G ;

Clegg, SM ;

Cobley, V ;

Cole, CG ;

Collier, RE ;

Connor, RE ;

Conroy, D ;

Corby, N ;

Coville, GJ ;

Cox, AV ;

Davis, J ;

Dawson, E ;

Dhami, PD ;

Dockree, C ;

Dodsworth, SJ ;

Durbin, RM ;

Ellington, A ;

Evans, KL ;

Fey, JM ;

Fleming, K ;

French, L .

NATURE, 1999, 402 (6761) :489-495

[4] Two function copies of the DGCR6 gene are present on human chromosome 22q11 due to a duplication of an ancestral locus [J].

Edelmann, L ;

Stankiewicz, P ;

Spiteri, E ;

Pandita, RK ;

Shaffer, L ;

Lupski, J ;

Morrow, BE .

GENOME RESEARCH, 2001, 11 (02) :208-217

[5] Regulatory RNAs [J].

Erdmann, VA ;

Barciszewska, MZ ;

Hochberg, A ;

de Groot, N ;

Barciszewski, J .

CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (07) :960-977

[6] A transcription map of the DiGeorge and velo-cardio-facial syndrome minimal critical 22q11 [J].

Gong, WK ;

Emanuel, BS ;

Collins, J ;

Kim, DH ;

Wang, ZL ;

Chen, F ;

Zhang, GZ ;

Roe, B ;

Budarf, ML .

HUMAN MOLECULAR GENETICS, 1996, 5 (06) :789-800

[7] The DNA sequence of human chromosome 21 [J].

Hattori, M ;

Fujiyama, A ;

Taylor, TD ;

Watanabe, H ;

Yada, T ;

Park, HS ;

Toyoda, A ;

Ishii, K ;

Totoki, Y ;

Choi, DK ;

Soeda, E ;

Ohki, M ;

Takagi, T ;

Sakaki, Y ;

Taudien, S ;

Blechschmidt, K ;

Polley, A ;

Menzel, U ;

Delabar, J ;

Kumpf, K ;

Lehmann, R ;

Patterson, D ;

Reichwald, K ;

Rump, A ;

Schillhabel, M ;

Schudy, A ;

Zimmermann, W ;

Rosenthal, A ;

Kudoh, J ;

Shibuya, K ;

Kawasaki, K ;

Asakawa, S ;

Shintani, A ;

Sasaki, T ;

Nagamine, K ;

Mitsuyama, S ;

Antonarakis, SE ;

Minoshima, S ;

Shimizu, N ;

Nordsiek, G ;

Hornischer, K ;

Brandt, P ;

Scharfe, M ;

Schön, O ;

Desario, A ;

Reichelt, J ;

Kauer, G ;

Blöcker, H ;

Ramser, J ;

Beck, A .

NATURE, 2000, 405 (6784) :311-319

[8] RNomics:: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse [J].

Hüttenhofer, A ;

Kiefmann, M ;

Meier-Ewert, S ;

O'Brien, J ;

Lehrach, H ;

Bachellerie, JP ;

Brosius, J .

EMBO JOURNAL, 2001, 20 (11) :2943-2953

[9] Noncoding RNA genes in dosage compensation and imprinting [J].

Kelley, RL ;

Kuroda, MI .

CELL, 2000, 103 (01) :9-12

[10]

Li M., 1994, American Journal of Human Genetics, V55, pA10

← 1 2 →