In vivo Imaging with a Fundus Camera in a Rat Modell of Laser-Induced Choroidal Neovascularization

被引：7

作者：

Cunea, Alexander ^{[1
]}

Meyer, Johanna ^{[1
]}

Russnnann, Christoph ^{[2
]}

Licha, Kai ^{[3
]}

Welker, Pia ^{[3
]}

Holz, Frank G. ^{[1
]}

Schrnitz-Valckenberg, Steffen ^{[1
]}

机构：

[1] Univ Bonn, Dept Ophthalmol, DE-53127 Bonn, Germany

[2] Carl Zeiss Meditec AG, Jena, Germany

[3] Mivenion GmbH, Berlin, Germany

来源：

OPHTHALMOLOGICA | 2014年 / 231卷 / 02期

关键词：

Fundus camera; Angiography; Reflectance; Animal model; Choroidal neovascularization; Fluorescein angiography; lndocyanine green angiography; Fundus photography; Polychromatic imaging; CELL APOPTOSIS; OPHTHALMOSCOPY; PHOTOGRAPHY;

D O I：

10.1159/000355094

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To investigate the use of imaging and quantitative measurement capabilities of a modified fundus camera in a rat model of laser-induced choroidal neovascularization. Methods: Following induction of experimental choroidal neovascularization, Dark Agouti rats underwent serial in vivo imaging with a fundus camera (FF450plus, Carl Zeiss MediTec, Jena, Germany), including color, reflectance and fluorescence imaging. Results: A custom-made setting allowed high-resolution imaging. Change of fluorescence intensity following intravenous or intravitreal dye injection could be quantitatively monitored overtime. Hardware binning resulted in an improved signal-to-noise ratio and a reduction of flash light intensity. Simultaneous fluorescence imaging following injection of two different dendritic polygylcerol sulfate dyes could be demonstrated. Conclusion: This study demonstrates the use and optimizations of a fundus camera for various in vivo imaging modalities in rats. Molecular imaging of the eye may allow for better insights into cellular dysfunction and optimization of therapeutic strategies. (C) 2013 S. Karger AG, Basel

引用

页码：117 / 123

页数：7

共 13 条

[1] Dendritic Polyglycerolsulfate Near Infrared Fluorescent (NIRF) Dye Conjugate for Non-Invasively Monitoring of Inflammation in an Allergic Asthma Mouse Model [J].