Limitations to oxygen transport and utilization during sprint exercise in humans: evidence for a functional reserve in muscle O2 diffusing capacity

被引:71
作者
Calbet, Jose A. L. [1 ,2 ]
Losa-Reyna, Jose [1 ,2 ]
Torres-Peralta, Rafael [1 ,2 ]
Rasmussen, Peter [3 ]
Gustavo Ponce-Gonzalez, Jesus [1 ,2 ]
Sheel, A. William [4 ]
de la Calle-Herrero, Jaime [1 ]
Guadalupe-Grau, Amelia [1 ,2 ]
Morales-Alamo, David [1 ,2 ]
Fuentes, Teresa [1 ]
Rodriguez-Garcia, Lorena [1 ]
Siebenmann, Christoph [3 ]
Boushel, Robert [4 ,5 ]
Lundby, Carsten [2 ]
机构
[1] Univ Las Palmas Gran Canaria, Dept Phys Educ, Las Palmas Gran Canaria 35017, Canary Island, Spain
[2] Univ Las Palmas Gran Canaria, Res Inst Biomed & Hlth Sci IUIBS, Las Palmas Gran Canaria 35016, Canary Island, Spain
[3] Univ Zurich, Inst Physiol, Ctr Integrat Human Physiol, Zurich, Switzerland
[4] Univ British Columbia, Sch Kinesiol, Vancouver, BC V5Z 1M9, Canada
[5] Swedish Sch Sport & Hlth Sci, Astrand Lab, Stockholm, Sweden
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2015年 / 593卷 / 20期
关键词
INTENSE DYNAMIC EXERCISE; ANAEROBIC ENERGY-RELEASE; SEVERE ACUTE-HYPOXIA; SKELETAL-MUSCLE; BLOOD-FLOW; METABOLISM; PERFUSION; ALTITUDE; RESPIRATION; PERFORMANCE;
D O I
10.1113/JP270408
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To determine the contribution of convective and diffusive limitations to (V) over dot(O2 peak) during exercise in humans, oxygen transport and haemodynamics were measured in 11 men (22 +/- 2years) during incremental (IE) and 30s all-out cycling sprints (Wingate test, WgT), in normoxia (Nx, P-IO2: 143mmHg) and hypoxia (Hyp, P-IO2: 73mmHg). Carboxyhaemoglobin (COHb) was increased to 6-7% before both WgTs to left-shift the oxyhaemoglobin dissociation curve. Leg (V) over dot(O2 peak) was measured by the Fick method and leg blood flow (BF) with thermodilution, and muscle O-2 diffusing capacity (D-MO2) was calculated. In the WgT mean power output, leg BF, leg O-2 delivery and leg O2 were 7, 5, 28 and 23% lower in Hyp than Nx (P<0.05); however, peak WgT D-MO2 was higher in Hyp (51.5 +/- 9.7) than Nx (20.5 +/- 3.0mlmin(-1)mmHg(-1), P<0.05). Despite a similar P-aO2 (33.3 +/- 2.4 and 34.1 +/- 3.3mmHg), mean capillary P-O2 (16.7 +/- 1.2 and 17.1 +/- 1.6mmHg), and peak perfusion during IE and WgT in Hyp, D-MO2 and leg (V) over dot(O2) were 12 and 14% higher, respectively, during WgT than IE in Hyp (both P<0.05). D-MO2 was insensitive to COHb (COHb: 0.7 vs. 7%, in IE Hyp and WgT Hyp). At exhaustion, the Y equilibration index was well above 1.0 in both conditions, reflecting greater convective than diffusive limitation to the O-2 transfer in both Nx and Hyp. In conclusion, muscle (V) over dot(O2) during sprint exercise is not limited by O-2 delivery, O-2 offloading from haemoglobin or structure-dependent diffusion constraints in the skeletal muscle. These findings reveal a remarkable functional reserve in muscle O-2 diffusing capacity.
引用
收藏
页码:4649 / 4664
页数:16
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