Assessment of Pollutant Reducing Effect by Poly-Culture and Bioremediation in Sediment of Marine Shrimp Ponds

Pollution assessment index analysis was used to evaluate pollutant reducing effect on shrimp pond bottom, by poly-culture of shrimp with oyster and clamworm respectively and applying two series of probiotics of SS3 (a composition of three strains Bacillus sp.) and MP (a composition of B.subtilis, B.cereus, and B. licheniformis) respectively. Results showed that poly-culture with a suitable density of culture species could reduce waste products in the shrimp pond, whereas high clamworm or oyster stocking density made for higher mortality and lower yield of shrimp than the control. Both clamworm density of 0.4 kg/m(2)-0.8 kg/m(2), which was calculated to be proportional to the relative pollution index (RI) as an exponential model, and oyster density of 0.32 kg/m(3)-0.64 kg/m(3) should be feasible to poly-culture based on this experiment. In the treatments of probiotics SS3, less effect of pollutant reducing and lower shrimp yields were observed. In contrast to SS3, the high MP applied treatments brought on not only higher shrimp survival or yield than the control, but also had successful bioremediation effects on the pond bottom. According to the value of the pollutant reduction index (PI) within the four techniques, the effect on pollution reduction was ranked as follows: poly-culture with clamworm, probiotics MP, poly-culture with oyster, probiotics SS3 (C) 2011 Published by Elsevier Ltd. Selection and/or peer-review under responsibility of Conference ESIAT2011 Organization Committee.