Rapid bioassay methods for Sr-90 in urine samples are needed to provide an early estimation of possible internal dose resulting from exposure to radiostrontium in the event of a radiological and nuclear emergency. In this work, a fast column separation method followed by liquid scintillation counting for detection of Sr-90 in urine was developed. Replicate spike and blank samples were analyzed for performance evaluation of the method. Using this method, a detection limit of similar to 10 Bq L-1 for Sr-90 can be achieved with a sample analysis turn-around time of 4 h for a set of 12 samples. The method is adequate to meet the radiobioassay acceptance criteria and is suitable for quick dose assessment of Sr-90 exposure following a radiation emergency.