A Standardized Method of Isolating Adipose-Derived Stem Cells for Clinical Applications

被引：38
|
作者：
Raposio, Edoardo [1 ,2 ]
Caruana, Giorgia [1 ,2 ]
Petrella, Maira [1 ,2 ]
Bonomini, Sabrina [3 ]
Grieco, Michele P. [1 ,2 ]
机构：
[1] Univ Parma, Dept Surg Sci, Div Plast Surg, I-43126 Parma, Italy
[2] Cutaneous Mininvas Regenerat & Plast Surg Unit, Bethesda, MD USA
[3] Parma Univ Hosp, Dept Clin & Expt Med, Div Hematol, Parma, Italy
来源：
ANNALS OF PLASTIC SURGERY | 2016年 / 76卷 / 01期
基金：
英国惠康基金; 美国国家卫生研究院;
关键词：
adipose-derived stem cells; regenerative medicine; collagenase; wound healing; TISSUE;
D O I：
10.1097/SAP.0000000000000609
中图分类号：
R61 [外科手术学];
学科分类号：
摘要：
White adipose tissue is the most abundant and accessible source of stem cells in the adult human body. In this paper, we present a standardised and safe method of isolating and maximizing the number of adipose-derived stem cells (ASCs) from conventional liposuction for clinical applications, which was carried out through both mechanical (centrifuge) and enzymatic (collagenase) means. Isolated cells were characterized through flow cytometry assay. Gathered data showed a greater amount (9.06 x 10(5) ASCs from 100 mL of adipose tissue) of isolated ASCs compared to previous protocol, also with high (99%) cell vitality; the procedure we presented is easy and fast (80 minutes), allowing collecting a significative number of mesenchymal stem cells, which can be used for clinical purposes, such as wound healing.
引用
收藏
页码：124 / 126
页数：3
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