DEVELOPMENT OF LC/MS/MS METHOD FOR DETERMINATION OF ACETAMINOPHEN METABOLITES

被引：0

作者：

Cesla, Petr ^{[1
]}

Rousar, Tomas ^{[2
]}

Nydlova, Erika ^{[1
,2
]}

Vrbova, Martina ^{[1
,2
]}

Ceslova, Lenka ^{[1
]}

Fischer, Jan ^{[1
]}

机构：

[1] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice, Czech Republic

[2] Univ Pardubice, Fac Chem Technol, Dept Biol & Biochem Sci, Pardubice, Czech Republic

来源：

CHEMICKE LISTY | 2013年 / 107卷

关键词：

TANDEM MASS-SPECTROMETRY; GLUTATHIONE CONJUGATE; QUANTIFICATION; REDUCTASE; PLASMA;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A liquid chromatography-tandem mass spectrometry method for the determination of two acetaminophen metabolites, i.e. acetaminophen-glutathione and acetaminophen-cysteine conjugates was developed. The octadecyl silica gel column has been used together with the gradient elution of methanol/water for fast separation of metabolites in less than four minutes. Tandem mass spectrometry in multiple reaction monitoring mode was applied for identification and quantitation of metabolites using deuterated acetaminophen as the internal standard.

引用

收藏

页码：S348 / S350

页数：3

相关论文

共 9 条

[1]

An J. H., 2012, BIOMED CHROMATOGR, V26, P1596

[2] Quantitative LC/MS/MS analysis of acetaminophen-cysteine adducts (APAP-CYS) and its application in acetaminophen overdose patients [J].

Hairin, T. ;

Marzilawati, A. R. ;

Didi, E. M. H. ;

Mahadeva, S. ;

Lee, Y. K. ;

Abd. Rahman, N. ;

Mustafa, A. M. ;

Chik, Z. .

ANALYTICAL METHODS, 2013, 5 (08) :1955-1964

[3] Comparison of inhibitory effects between acetaminophen-glutathione conjugate and reduced glutathione in human glutathione reductase [J].

Nydlova, Erika ;

Vrbova, Martina ;

Cesla, Petr ;

Jankovicova, Barbora ;

Ventura, Karel ;

Rousar, Tomas .

JOURNAL OF APPLIED TOXICOLOGY, 2014, 34 (09) :968-973

[4] Analysis of acetaminophen glucuronide conjugate accompanied by adduct ion production by liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry [J].

Ohta, M ;

Kawakami, N ;

Yamato, S ;

Shimada, K .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2003, 30 (06) :1759-1764

[5] Purified Acetaminophen-Glutathione Conjugate Is Able To Induce Oxidative Stress in Rat Liver Mitochondria [J].

Rousar, T. ;

Nydlova, E. ;

Cesla, P. ;

Stankova, P. ;

Kucera, O. ;

Parik, P. ;

Cervinkova, Z. .

PHYSIOLOGICAL RESEARCH, 2012, 61 :S103-S109

[6] Glutathione Reductase Is Inhibited by Acetaminophen-glutathione Conjugate In Vitro [J].

Rousar, T. ;

Parik, P. ;

Kucera, O. ;

Bartos, M. ;

Cervinkova, Z. .

PHYSIOLOGICAL RESEARCH, 2010, 59 (02) :225-232

[7] Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography-tandem mass spectrometry [J].

Taylor, Rachel R. ;

Hoffman, Keith L. ;

Schniedewind, Bjoern ;

Clavijo, Claudia ;

Galinkin, Jeffrey L. ;

Christians, Uwe .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2013, 83 :1-9

[8] Quantification of acetaminophen and two of its metabolites in human plasma by ultra-high performance liquid chromatography-low and high resolution tandem mass spectrometry [J].

Tonoli, David ;

Varesio, Emmanuel ;

Hopfgartner, Gerard .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2012, 904 :42-50

[9] Screening and characterization of reactive metabolites using glutathione ethyl ester in combination with Q-trap mass spectrometry [J].

Wen, Bo ;

Fitch, William L. .

JOURNAL OF MASS SPECTROMETRY, 2009, 44 (01) :90-100