Antidermatitic perspective of hydrocortisone as chitosan nanocarriers: An ex vivo and in vivo assessment using an NC/Nga mouse model

被引:36
作者
Hussain, Zahid [1 ]
Katas, Haliza [1 ]
Amin, Mohd Cairul Iqbal Mohd [1 ]
Kumulosasi, Endang [1 ]
Sahudin, Shariza [1 ]
机构
[1] Univ Kebangsaan Malaysia, Fac Pharm, Ctr Drug Delivery Res, Kuala Lumpur 50300, Malaysia
关键词
Corticosteroids; allergic contact dermatitis; percutaneous; biodegradable polymers; nanoparticles; in vitro model; ex vivo permeation; in vivo efficacy; PENETRATION ENHANCERS; TRANSDERMAL DELIVERY; SKIN PENETRATION; WATER-LOSS; DERMATITIS; NANOPARTICLES; ACCUMULATION; ULTRASOUND; SYSTEMS;
D O I
10.1002/jps.23446
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The aim of this study to administer hydrocortisone (HC) percutaneously in the form of polymeric nanoparticles (NPs) to alleviate its transcutaneous absorption, and to derive additional wound-healing benefits of chitosan. HC-loaded NPs had varied particle sizes, zeta potentials, and entrapment efficiencies, when drug-to-polymer mass ratios increased from 1:1 to 1:8. Ex vivo permeation analysis showed that the nanoparticulate formulation of HC significantly reduced corresponding flux [approximate to 24 mu g/(cm2 h)] and permeation coefficient (approximate to 4.8 x 103 cm/h) of HC across the full thickness NC/Nga mouse skin. The nanoparticulate formulation also exhibited a higher epidermal (1610 +/- 42 mu g/g of skin) and dermal (910 +/- 46 mu g/g of skin) accumulation of HC than those associated with control groups. An in vivo assessment using an NC/Nga mouse model further revealed that mice treated with the nanoparticulate system efficiently controlled transepidermal water loss [15 +/- 2 g/(m2 h)], erythema intensity (232 +/- 12), dermatitis index (mild), and thickness of skin (456 +/- 27 mu m). Taken together, histopathological examination predicted that the nanoparticulate system showed a proficient anti-inflammatory and antifibrotic activity against atopic dermatitic (AD) lesions. Our results strongly suggest that HC-loaded NPs have promising potential for topical/transdermal delivery of glucocorticoids in the treatment of AD. (c) 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:10631075, 2013
引用
收藏
页码:1063 / 1075
页数:13
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