Applicability of major histocompatibility complex DRB1 alleles as markers to detect vertebrate hybridization: a case study from Iberian ibex x domestic goat in southern Spain

被引:19
作者
Alasaad, Samer [1 ,2 ]
Fickel, Joerns [3 ]
Rossi, Luca [4 ]
Sarasa, Mathieu [5 ]
Benitez-Camacho, Buenaventura
Granados, Jose E. [6 ]
Soriguer, Ramon C. [1 ,2 ]
机构
[1] CSIC, Estn Biol Donana, Seville 41092, Spain
[2] Univ Zurich, Inst Evolutionary Biol & Environm Studies IEU, CH-8057 Zurich, Switzerland
[3] Leibniz Inst Zoo & Wildlife Res, Res Grp Evolutionary Genet, D-10315 Berlin, Germany
[4] Univ Turin, Dipartimento Prod Anim Epidemiol & Ecol, I-10095 Grugliasco, Italy
[5] Grp Biol Especies Cineget & Plagas RNM 118, Seville 41092, Spain
[6] Espacio Nat Sierra Nevada, Cordoba, Spain
关键词
Hybridization; MHC alleles; Capra pyrenaica; Capra hircus; Wildlife conservation; INTROGRESSIVE HYBRIDIZATION; MICROSATELLITE MARKERS; MITOCHONDRIAL-DNA; CAPRA-PYRENAICA; WILD; DIVERSITY; BIOLOGY; GENES; RECOMBINATION; POPULATIONS;
D O I
10.1186/1751-0147-54-56
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Background: Hybridization between closely related wild and domestic species is of great concern because it can alter the evolutionary integrity of the affected populations. The high allelic variability of Major Histocompatibility Complex (MHC) loci usually excludes them from being used in studies to detect hybridization events. However, if a) the parental species don't share alleles, and b) one of the parental species possesses an exceptionally low number of alleles (to facilitate analysis), then even MHC loci have the potential to detect hybrids. Results: By genotyping the exon2 of the MHC class II DRB1 locus, we were able to detect hybridization between domestic goats (Capra hircus) and free-ranging Iberian ibex (Capra pyrenaica hispanica) by molecular means. Conclusions: This is the first documentation of a Capra pyrenaica x Capra hircus hybridization, which presented us the opportunity to test the applicability of MHC loci as new, simple, cost-effective, and time-saving approach to detect hybridization between wild species and their domesticated relatives, thus adding value to MHC genes role in animal conservation and management.
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页数:6
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