The aim of the conducted research was to determine the effect of biostimulators on the content of glycoalkaloids in leaves and tubers of three edible potato varieties. The experiment was established in a split-plot system in three replications. The examined factors were: I - three varieties of edible potato: Honorata, Jelly, Tajfun, and II - five types of using biostimulators: biostimulator BrunatneBio Zloto Cytokinin (R), GreenOk (R), Kelpak SL (R), Titanit (R), control variant. In the biostimulators group there were products containing functional elements, eg titanium, products based on algae extracts, and products containing amino acids. The lowest concentration of glycoalkaloids in leaves and tubers of edible potato was found in the Jelly variety -251 mg.kg(-1) on average in leaves, and -80.5 mg.kg(-1) in tubers, while the largest in the Tajfun variety - on average in leaves 370 mg.kg(-1), in tubers 110 mg.kg(-1). Under the influence of all bioregulators, there was a significant reduction in the content of harmful glycosides as compared to the control variant. The lowest concentration was recorded under the influence of the BrunatneBio Zloto biostimulator - on average in the leaves 293 mg.kg(-1), in tubers 91 mg.kg(-1).