Microbial Dynamics of Polyhydroxyalkanoates Production from Waste Glycerol using RISA Technique

被引：0

作者：

Suwimon, Kanchanasuta ^{[1
]}

Nipon, Pisutpaisal ^{[2
,3
,4
]}

机构：

[1] Mahidol Univ, Fac Publ Hlth, Dept Environm Hlth Sci, Bangkok 10400, Thailand

[2] King Mongkuts Univ Technol North Bangkok, Fac Sci Appl, Dept Agroind Technol, Bangkok 10800, Thailand

[3] King Mongkuts Univ Technol North Bangkok, Biosensor & Bioelect Technol Ctr, Bangkok 10800, Thailand

[4] King Mongkuts Univ Technol North Bangkok, Res & Technol Ctr Renewable Prod & Energy, Bangkok 10800, Thailand

来源：

RESEARCH JOURNAL OF BIOTECHNOLOGY | 2015年 / 10卷 / 10期

关键词：

Polyhydroxyalkanoate; glycerol waste; mixed culture; Ribosomal intergenic spacer analysis; COMMUNITY STRUCTURE; IDENTIFICATION;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ribosomal intergenic spacer analysis (RISA) technique was used to analyze the dynamics of microbial community capable of converting waste glycerol to polyhydroxyalkanoates (PHA) under cultivation on 5% nu.nu(-1) crude glycerol as a carbon source. Three dominant band patterns were observed during 55 days cultivation. 16S rRNA sequences of dominant RISA bands showed bacterial community cultivated in waste glycerol were closely related to Azoarcus sp., Bacillus cereus, Bacillus pseudofirmus, Flavobacterium columnareand Thauera sp. The RISA technique could be used to identify dynamic microbial community in the bioreactor.

引用

页码：69 / 73

页数：5

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