Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography

被引：61
作者：
Fu, Qing [1 ,2 ]
Guo, Zhimou [2 ]
Zhang, Xiuli [2 ]
Liu, Yanfang [2 ]
Liang, Xinmiao [1 ,2 ]
机构：
[1] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
[2] Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
来源：
JOURNAL OF SEPARATION SCIENCE | 2012年 / 35卷 / 14期
基金：
美国国家科学基金会;
关键词：
Characterization; HILIC; RP; Stevia rebaudiana; Two-dimensional liquid chromatography (2D-LC); FLUID EXTRACTION; GLYCOSIDES; STEVIOSIDE; SEPARATION; SWEETENER; PEPTIDES; SYSTEM; LEAVES;
D O I：
10.1002/jssc.201101103
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2D-RPLC/HILIC) system was successfully applied for comprehensive characterization of steviol glycosides from Stevia rebaudiana. The experiments were performed in offline mode using an XCharge C18 column in first dimension and an XAmide column in second dimension. In first dimension, preliminary separation of Stevia aqueous extract was accomplished and 30 fractions were collected. Then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. Comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. As a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. The result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in Stevia. This 2D-RPLC/HILIC method will be a promising tool for the purification of low-abundance compounds from natural products.
引用
收藏
页码：1821 / 1827
页数：7
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