Zirex: a Novel Zinc-Regulated Expression System for Lactococcus lactis

被引：33
|
作者：
Mu, Dongdong [1 ]
Montalban-Lopez, Manuel [1 ,2 ]
Masuda, Yoshimitsu [1 ]
Kuipers, Oscar P. [1 ,2 ]
机构：
[1] Univ Groningen, Dept Mol Genet, Groningen, Netherlands
[2] Kluyver Ctr Genom Ind Fermentat, Groningen, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2013年 / 79卷 / 14期
关键词：
CONTROLLED GENE-EXPRESSION; ACID BACTERIA; STREPTOCOCCUS-PNEUMONIAE; NISIN; GROWTH; CREMORIS; SEQUENCE; PROTEIN; CLONING;
D O I：
10.1128/AEM.00866-13
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and P-czcD. P-czcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.
引用
收藏
页码：4503 / 4508
页数：6
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