Immobilized Aspergillus niger Lipase with SiO2 Nanoparticles in Sol-Gel Materials

被引：12

作者：

Xu, Li ^{[1
]}

Ke, Caixia ^{[1
]}

Huang, Ying ^{[2
]}

Yan, Yunjun ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Peoples R China

[2] Huazhong Univ Sci & Technol, Shenzhen R&D Ctr, Shenzhen 518060, Peoples R China

来源：

CATALYSTS | 2016年 / 6卷 / 10期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

sol-gel; SiO2; nanoparticles; lipase; immobilization; stability; CANDIDA-CYLINDRACEA; ENCAPSULATION; ENANTIOSELECTIVITY; ENTRAPMENT; HYDROLYSIS; SUPPORT;

D O I：

10.3390/catal6100149

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Lipase from Aspergillus niger was "doubly immobilized" with SiO2 nanoparticles in sol-gel powders prepared via the base-catalyzed polymerization of tetramethoxysilane (TMOS) and methyltreimethoxysilane (MTMS). The hydrolytic activity of the immobilized lipase was measured using the p-nitrophenyl palmitate hydrolysis method. The results showed that the optimum preparation conditions for the gels were made using a MTMS/TMOS molar ratio of 5, 60 mg of SiO2 nanoparticles, a water/silane molar ratio of 12, 120 mg of enzyme supply, and 120 mu L of PEG400. Under the optimal conditions, the immobilized lipase retained 92% of the loading protein and 94% of the total enzyme activity. Characteristic tests indicated that the immobilized lipase exhibited much higher thermal and pH stability than its free form, which shows great potential for industrial applications.

引用

页数：12

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