Immobilized Aspergillus niger Lipase with SiO2 Nanoparticles in Sol-Gel Materials

被引：12
|
作者：
Xu, Li [1 ]
Ke, Caixia [1 ]
Huang, Ying [2 ]
Yan, Yunjun [1 ,2 ]
机构：
[1] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Peoples R China
[2] Huazhong Univ Sci & Technol, Shenzhen R&D Ctr, Shenzhen 518060, Peoples R China
来源：
CATALYSTS | 2016年 / 6卷 / 10期
基金：
中国国家自然科学基金; 国家高技术研究发展计划(863计划);
关键词：
sol-gel; SiO2; nanoparticles; lipase; immobilization; stability; CANDIDA-CYLINDRACEA; ENCAPSULATION; ENANTIOSELECTIVITY; ENTRAPMENT; HYDROLYSIS; SUPPORT;
D O I：
10.3390/catal6100149
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Lipase from Aspergillus niger was "doubly immobilized" with SiO2 nanoparticles in sol-gel powders prepared via the base-catalyzed polymerization of tetramethoxysilane (TMOS) and methyltreimethoxysilane (MTMS). The hydrolytic activity of the immobilized lipase was measured using the p-nitrophenyl palmitate hydrolysis method. The results showed that the optimum preparation conditions for the gels were made using a MTMS/TMOS molar ratio of 5, 60 mg of SiO2 nanoparticles, a water/silane molar ratio of 12, 120 mg of enzyme supply, and 120 mu L of PEG400. Under the optimal conditions, the immobilized lipase retained 92% of the loading protein and 94% of the total enzyme activity. Characteristic tests indicated that the immobilized lipase exhibited much higher thermal and pH stability than its free form, which shows great potential for industrial applications.
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页数：12
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