Improved Sendai viral system for reprogramming to naive pluripotency

被引：26

作者：

Kunitomi, Akira ^{[1
,2
]}

Hirohata, Ryoko ^{[1
,3
]}

Arreola, Vanessa ^{[2
]}

Osawa, Mitsujiro ^{[1
,7
]}

Kato, Tomoaki M. ^{[1
,3
]}

Nomura, Masaki ^{[1
,3
]}

Kawaguchi, Jitsutaro ^{[4
]}

Hara, Hiroto ^{[4
]}

Kusano, Kohji ^{[4
]}

Takashima, Yasuhiro ^{[1
]}

Takahashi, Kazutoshi ^{[1
]}

Fukuda, Keiichi ^{[5
]}

Takasu, Naoko ^{[1
,3
]}

Yamanaka, Shinya ^{[1
,2
,3
,6
]}

机构：

[1] Kyoto Univ, Ctr iPS Cell Res & Applicat CiRA, Kyoto 6068507, Japan

[2] Gladstone Inst Cardiovasc Dis, San Francisco, CA 94158 USA

[3] CiRA Fdn, Kyoto 6068397, Japan

[4] ID Pharma Co Ltd, Ibaraki 3002611, Japan

[5] Keio Univ, Dept Cardiol, Sch Med, Tokyo 1608582, Japan

[6] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94143 USA

[7] Thyas Co Ltd, Kyoto 6068501, Japan

来源：

CELL REPORTS METHODS | 2022年 / 2卷 / 11期

基金：

美国国家卫生研究院;

关键词：

STEM-CELLS; GENERATION; VIRUS; BLASTOCYST; INDUCTION; PACKAGE; STATES; MOUSE;

D O I：

10.1016/j.crmeth.2022.100317

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Naive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified method can be applied not only to fibroblasts but also to other somatic cell types. SeV vectors disappear quickly at early passages, and this approach enables the generation of naive iPSCs in a feeder-free culture. The naive iPSCs generated by this method show better differentiation to trilineage and extra-embryonic trophectoderm than those derived by conventional methods. This method can expand the application of iPSCs to research on early human development and regenerative medicine.

引用

页数：16

共 45 条

[1]

[Anonymous], CELL, V158, P1254

[2] Minfi: a flexible and comprehensive Bioconductor package for the analysis of Infinium DNA methylation microarrays [J].

Aryee, Martin J. ;

Jaffe, Andrew E. ;

Corrada-Bravo, Hector ;

Ladd-Acosta, Christine ;

Feinberg, Andrew P. ;

Hansen, Kasper D. ;

Irizarry, Rafael A. .

BIOINFORMATICS, 2014, 30 (10) :1363-1369

[3] Derivation of consensus inactivation status for X-linked genes from genome-wide studies [J].

Balaton, Bradley P. ;

Cotton, Allison M. ;

Brown, Carolyn J. .

BIOLOGY OF SEX DIFFERENCES, 2015, 6

[4] Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors [J].

Ban, Hiroshi ;

Nishishita, Naoki ;

Fusaki, Noemi ;

Tabata, Toshiaki ;

Saeki, Koichi ;

Shikamura, Masayuki ;

Takada, Nozomi ;

Inoue, Makoto ;

Hasegawa, Mamoru ;

Kawamata, Shin ;

Nishikawa, Shin-Ichi .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (34) :14234-14239

[5] The tumorigenicity of human embryonic and induced pluripotent stem cells [J].

Ben-David, Uri ;

Benvenisty, Nissim .

NATURE REVIEWS CANCER, 2011, 11 (04) :268-277

[6] Reference Maps of Human ES and iPS Cell Variation Enable High-Throughput Characterization of Pluripotent Cell Lines [J].

Bock, Christoph ;

Kiskinis, Evangelos ;

Verstappen, Griet ;

Gu, Hongcang ;

Boulting, Gabriella ;

Smith, Zachary D. ;

Ziller, Michael ;

Croft, Gist F. ;

Amoroso, Mackenzie W. ;

Oakley, Derek H. ;

Gnirke, Andreas ;

Eggan, Kevin ;

Meissner, Alexander .

CELL, 2011, 144 (03) :439-452

[7] Origins and implications of pluripotent stem cell variability and heterogeneity [J].

Cahan, Patrick ;

Daley, George Q. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2013, 14 (06) :357-368

[8] Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells [J].

Di Stefano, Bruno ;

Ueda, Mai ;

Sabri, Shan ;

Brumbaugh, Justin ;

Huebner, Aaron J. ;

Sahakyan, Anna ;

Clement, Kendell ;

Clowers, Katie J. ;

Erickson, Alison R. ;

Shioda, Keiko ;

Gygi, Steven P. ;

Gu, Hongcang ;

Shioda, Toshi ;

Meissner, Alexander ;

Takashima, Yasuhiro ;

Plath, Kathrin ;

Hochedlinger, Konrad .

NATURE METHODS, 2018, 15 (09) :732-+

[9] STAR: ultrafast universal RNA-seq aligner [J].

Dobin, Alexander ;

Davis, Carrie A. ;

Schlesinger, Felix ;

Drenkow, Jorg ;

Zaleski, Chris ;

Jha, Sonali ;

Batut, Philippe ;

Chaisson, Mark ;

Gingeras, Thomas R. .

BIOINFORMATICS, 2013, 29 (01) :15-21

[10] Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome [J].

Fusaki, Noemi ;

Ban, Hiroshi ;

Nishiyama, Akiyo ;

Saeki, Koichi ;

Hasegawa, Mamoru .

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES, 2009, 85 (08) :348-362

← 1 2 3 4 5 →