Fluxes of Na+, K+ and Cl-, and osmotic adjustment in Lycopersicon pimpinellifolium and L-esculentum during short- and long-term exposures to NaCl

NaCl (140 mM) was applied to 14-day-old plants of salt-sensitive Lycopersicon esculentum Mill. cv. Volgogradskij and its wild relative L. pimpinellifolium Mill. accession PE-2. Changes in the relative growth rate of whole plant, and in the levels of inorganic and organic solutes in leaves, stems and roots were followed for 15 days after the application. Short-term salt exposure (4-6 days of salinization) resulted in enhanced relative growth rates for L. pimpinellifolium, but did not affect growth of L. esculentum. After 6 days of salinization, the relative growth rates of both species decreased significantly; leading to practically comparable growth rates for them by day 15. In all parts of both species, the contribution of organic solutes to the osmotic potential (Psi(s)) gradually decreased from 30% on day 0 to a value lower than 5% on day 4. Int. pimpinellifolium, compared to L. esculentum, short-term salt exposure resulted in (1) a higher percentage of adjustment of Psi(s); and (2) increases in Na+ and K+ uptake rates, and in the levels of organic acids and proline (the level of which reached that of sugars, i.e., 10 pmol g(-1) dry weight. Conversely, in L. esculentum, drastic reductions of K+ uptake rates and organic acid levels occurred already on day 1. During long-term salt exposure, both species were able to adjust osmotically and both exhibited decreases in organic acid levels as well as in K+ uptake and accumulation rates in all parts. The results are discussed in an attempt to explain the adaptive responses during short-term salt exposure and the metabolic dysfunctions that lead to growth decrease after longterm exposure to salt.