Duplex real-time polymerase chain reaction for simultaneous detection and quantification of Anaplasma marginale and Anaplasma centrale

被引：42

作者：

Decaro, Nicola ^{[1
]}

Carelli, Grazia ^{[2
]}

Lorusso, Eleonora ^{[1
,2
]}

Lucente, Maria Stella ^{[1
]}

Greco, Grazia ^{[1
]}

Lorusso, Alessio ^{[1
]}

Radogna, Ariatiria ^{[1
,2
]}

Ceci, Luigi ^{[2
]}

Buonavoglia, Canio ^{[1
]}

机构：

[1] Univ Bari, Dept Publ Hlth & Anim Sci, Fac Vet Med, I-70010 Bari, Italy

[2] Univ Bari, Dept Anim Hlth & Wellbeing, Fac Vet Med, I-70010 Bari, Italy

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2008年 / 20卷 / 05期

关键词：

Anaplasma centrale; Anaplasma marginale; cattle; duplex real-time polymerase chain reaction;

D O I：

10.1177/104063870802000511

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Anaplasma marginale and Anaplasma centrale are rickettsial pathogens responsible for acute disease and mild infections, respectively, in cattle herds. A duplex real-time polymerase chain reaction (PCR) assay with probes labeled with different fluorophores was developed for simultaneous detection and quantification of A. marginale and A. centrale DNA in bovine blood samples. The assay was able to detect as few as 10(1) and 10(2) DNA copies for A. marginale and A. centrale, respectively, with optimal specificity and reproducibility. Analysis by real-time and nested PCR carried out oil 54 samples previously tested by reverse line blot hybridization showed that the established duplex real-time PCR assay can detect and quantify the 2 Anaplasma spp., even if present simultaneously in the same blood samples. Such an assay could be used in pathogenesis studies oil bovine acute anaplasmosis.

引用

页码：606 / 611

页数：6

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