The complete amino acid sequence of human placental oxytocinase

被引：51
作者：
Laustsen, PG
Rasmussen, TE
Petersen, K
PedrazaDiaz, S
Moestrup, SK
Gliemann, J
SottrupJensen, L
Kristensen, T
机构：
[1] AARHUS UNIV,DEPT CHEM,DEPT BIOL MOL & STRUCT,DK-8000 AARHUS,DENMARK
[2] AARHUS UNIV,DEPT MED BIOCHEM,DK-8000 AARHUS,DENMARK
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1997年 / 1352卷 / 01期
关键词：
zinc metallopeptidase; aminopeptidase; oxytocinase; placenta; heart; muscle;
D O I：
10.1016/S0167-4781(97)00036-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complete amino acid sequence of human placental oxytocinase (placental leucine aminopeptidase) has been determined by cDNA cloning and sequencing. Oxytocinase is a type II integral membrane protein of 1025 amino acid residues, consisting of an acidic intracellular region of 110 amino acids followed by a hydrophobic transmembrane segment of 22 residues and 893 extracellular residues containing the characteristic Zn2+ coordination sequence element His-Glu-Xaa-Xaa-His-(18 residues)-Glu found in gluzincins. Two sets of cDNA clones with different 5'-ends were isolated and suggested to represent different spliced products of 3.6 kb (mature mRNA) and 12 kb, respectively. Oxytocinase mRNA is present in large amounts in placenta, heart and skeletal muscle and in small amounts in brain, kidney, liver and pancreas. A conserved sequence element, the GAMEN motif, which distinguishes the aminopeptidase family among gluzincins from other gluzincins, has been identified.
引用
收藏
页码：1 / 7
页数：7
相关论文
共 30 条
[21]   COMPLETE AMINO-ACID SEQUENCE OF HUMAN INTESTINAL AMINOPEPTIDASE-N AS DEDUCED FROM CLONED CDNA [J].
OLSEN, J ;
COWELL, GM ;
KONIGSHOFER, E ;
DANIELSEN, EM ;
MOLLER, J ;
LAUSTSEN, L ;
HANSEN, OC ;
WELINDER, KG ;
ENGBERG, J ;
HUNZIKER, W ;
SPIESS, M ;
SJOSTROM, H ;
NOREN, O .
FEBS LETTERS, 1988, 238 (02) :307-314
[22]   Human placental leucine aminopeptidase oxytocinase - A new member of type II membrane-spanning zinc metallopeptidase family [J].
Rogi, T ;
Tsujimoto, M ;
Nakazato, H ;
Mizutani, S ;
Tomoda, Y .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (01) :56-61
[23]   DECREASED LEVELS OF AMNIOTIC-FLUID OXYTOCINASE ACTIVITY IN PREECLAMPSIA [J].
ROY, AC ;
VIEGAS, OAC ;
SEN, DK ;
RATNAM, SS .
GYNECOLOGIC AND OBSTETRIC INVESTIGATION, 1993, 35 (03) :166-168
[24]   CLONING OF A CDNA-ENCODING AN ECTOENZYME THAT DEGRADES THYROTROPIN-RELEASING-HORMONE [J].
SCHAUDER, B ;
SCHOMBURG, L ;
KOHRLE, J ;
BAUER, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (20) :9534-9538
[25]  
TSUJIMOTO M, 1995, ARCH BIOCHEM BIOPHYS, V292, P388
[26]   Identification of glutamate residues essential for catalytic activity and zinc coordination in aminopeptidase A [J].
Vazeux, G ;
Wang, JY ;
Corvol, P ;
LlorensCortes, C .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (15) :9069-9074
[27]   HISTIDINE RESIDUE IN THE ZINC-BINDING MOTIF OF AMINOPEPTIDASE-A IS CRITICAL FOR ENZYMATIC-ACTIVITY [J].
WANG, JY ;
COOPER, MD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1222-1226
[28]  
WATT VM, 1989, J BIOL CHEM, V264, P5480
[29]   MOLECULAR-CLONING OF THE MURINE BP-1/6C3 ANTIGEN - A MEMBER OF THE ZINC-DEPENDENT METALLOPEPTIDASE FAMILY [J].
WU, Q ;
LAHTI, JM ;
AIR, GM ;
BURROWS, PD ;
COOPER, MD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (03) :993-997
[30]   COMPLETE SEQUENCE OF RABBIT KIDNEY AMINOPEPTIDASE-N AND MESSENGER-RNA LOCALIZATION IN RABBIT KIDNEY BY IN-SITU HYBRIDIZATION [J].
YANG, XF ;
MILHIET, PE ;
GAUDOUX, F ;
CRINE, P ;
BOILEAU, G .
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1993, 71 (5-6) :278-287
123