Hypoglycemic and hypolipidemic activity of aqueous leaf extract of Passiflora suberosa L

被引:29
作者
Sudasinghe, Hasani Prabodha [1 ]
Peiris, Dinithi C. [1 ]
机构
[1] Univ Sri Jayewardenepura, Dept Zool, Ctr Plant Mat & Herbal Prod Res, Nugegoda, Western Provinc, Sri Lanka
来源
PEERJ | 2018年 / 6卷
关键词
Passiflora suberosa; Aqueous leaf extract; Hypoglycemia; Hypercholesterolemia; ANTIDIABETIC ACTIVITY; MICE; HYPERGLYCEMIA; ANTIOXIDANT; LEAVES; CELLS; LIVER;
D O I
10.7717/peerj.4389
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Leaves of Passiflora suberosa L. (Family: Passifloraceae; common name: wild passion fruit, devil's pumpkin) are used in Sri Lankan traditional medicine for treating diabetes. The present study, investigated the in vivo ability of P. suberosa leaves to manage blood sugar status and associated cholesterol levels. Mechanisms of action and toxicity were also determined. Phytochemical screening of aqueous' extracts of P. suberosa leaves and carbohydrate content of the leaves were determined according to previously published methods. In two group of male mice (n = 9), effects on fasting randomand blood glucose levels (BGLs) of different acute doses (0, 25, 50, 100 and 200 mg/kg) of the aqueous leaf extract (ALE) were evaluated at 1, 3, and 5 h post-treatment, In another set of mice, the fasting BGL was evaluated following treatment of 0 or 50 mg/kg ALE (dose prescribed in traditional medicine) for 30 consecutive days, The lipid profile, some mechanism of ALE action (diaphragm glucose uptake, glycogen content in the liver and skeletal muscles) and its toxicity (behavioural observation, food and water intake, hepatoxicity) were also assessed following 30-day treatment. However, sucrose and glucose tolerance tests and intestinal glucose uptake were conducted to determine portion of mechanisms of action following single dose of 50 mg/kg ALE. Phytochemi cal screening revealed the presence of alkaloids, unsaturated sterols, triterpenes, saponins, flavonoids, tannins and proanthocyanidins. Carbohydrate content of the leaves was 12.97%. The maximum hypoglycemic effect was observed after 4 h of 50 and 100 mg/kg ALE administration. The extract decreased fasting BGL (18%) following an oral sucrose challenge and inhibited (79%) glucose absorption from the intestine. Correspondingly, the levels of glycogen in the liver (61%) and in the skeletal muscles (57%) were found be higher than that of the control group. The levels of total cholesterol (17%) and tri_oyceraidehyde levels (12%) found to be reduced in treated groups. Furthermore, no significant toxic effects were observed in treated groups. The present results suggest that the leaves ofP, suberosa can be used to manage blood glucose and cholesterol levels. Isolation of active compounds are recommended for further analysis.
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页数:17
相关论文
共 47 条
  • [1] In vitro hypoglycemic effects of selected dietary fiber sources
    Ahmed, Faiyaz
    Sairam, Sudha
    Urooj, Asna
    [J]. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, 2011, 48 (03): : 285 - 289
  • [2] Hypoglycemic effect of extracts and fractions from Psacalium decompositum in healthy and alloxan-diabetic mice
    Alarcon-Aguilar, FJ
    Jimenez-Estrada, M
    Reyes-Chilpa, R
    Roman-Ramos, R
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2000, 72 (1-2) : 21 - 27
  • [3] Enhanced insulin sensitivity in skeletal muscle and liver by physiological overexpression of SIRT6
    Anderson, Jason G.
    Ramadori, Giorgio
    Ioris, Rafael M.
    Galie, Mirco
    Berglund, Eric D.
    Coate, Katie C.
    Fujikawa, Teppei
    Pucciarelli, Stefania
    Moreschini, Benedetta
    Amici, Augusto
    Andreani, Cristina
    Coppari, Roberto
    [J]. MOLECULAR METABOLISM, 2015, 4 (11): : 846 - 856
  • [4] [Anonymous], 1998, BUSINESS MEDIA, DOI DOI 10.1007/978-94-009-5570-7
  • [5] [Anonymous], 2017, Diabetes
  • [6] [Anonymous], 2017, GLOBAL REPORT DIABET
  • [7] Kolaviron, a Garcinia biflavonoid complex ameliorates hyperglycemia-mediated hepatic injury in rats via suppression of inflammatory responses
    Ayepola, Omolola R.
    Chegou, Novel N.
    Brooks, Nicole L.
    Oguntibeju, Oluwafemi O.
    [J]. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2013, 13
  • [8] Ballinger A., 2011, Essentials of kumar and clark's clinical medicine E-book. Vol, V5th
  • [9] BANDARA S, 2016, BEAT DIABETES SRI LA
  • [10] Preventive effects of Salvia officinalis leaf extract on insulin resistance and inflammation in a model of high fat diet-induced obesity in mice that responds to rosiglitazone
    Ben Khedher, Mohamed R.
    Hammami, Mohamed
    Arch, Jonathan R. S.
    Hislop, David C.
    Eze, Dominic
    Wargent, Edward T.
    Kepczynska, Magorzata A.
    Zaibi, Mohamed S.
    [J]. PEERJ, 2018, 6