Imaging individual mRNA molecules using multiple singly labeled probes

被引：1271

作者：

Raj, Arjun ^{[1
]}

van den Bogaard, Patrick ^{[2
]}

Rifkin, Scott A. ^{[1
]}

van Oudenaarden, Alexander ^{[1
]}

Tyagi, Sanjay ^{[2
]}

机构：

[1] MIT, Dept Phys, Cambridge, MA 02139 USA

[2] Univ Med & Dent New Jersey, New Jersey Med Sch, Publ Hlth Res Inst Ctr, Newark, NJ 07103 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 10期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1038/NMETH.1253

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method for imaging individual mRNA molecules in fixed cells by probing each mRNA species with 48 or more short, singly labeled oligonucleotide probes. This makes each mRNA molecule visible as a computationally identifiable fluorescent spot by fluorescence microscopy. We demonstrate simultaneous detection of three mRNA species in single cells and mRNA detection in yeast, nematodes, fruit fly wing discs, and mammalian cell lines and neurons.

引用

页码：877 / 879

页数：3

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