Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics

被引：18

作者：

Hazeki, Kaoru ^{[1
]}

Nigorikawa, Kiyomi ^{[1
]}

Takaba, Yuki ^{[1
]}

Segawa, Tomohiro ^{[2
]}

Nukuda, Akiko ^{[2
]}

Masuda, Ayaka ^{[2
]}

Ishikawa, Yuki ^{[2
]}

Kubota, Koji ^{[1
]}

Takasuga, Shunsuke ^{[3
]}

Hazeki, Osamu ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Hiroshima 7348553, Japan

[2] Hiroshima Univ, Fac Pharmaceut Sci, Hiroshima 7348553, Japan

[3] Akita Univ, Sch Med, Dept Pathol & Immunol, Akita 0108543, Japan

来源：

FEBS LETTERS | 2012年 / 586卷 / 22期

基金：

日本学术振兴会;

关键词：

IgG-opsonized red blood cell; Phagosome maturation; Phosphoinositide 3 '-phosphatase; PTEN; PIKfyve; RAW264.7; macrophage; RECEPTOR-MEDIATED PHAGOCYTOSIS; PHOSPHOINOSITIDE; 3-KINASE; CLASS-III; CLASS-I; PHOSPHATASES; ACTIVATION; MATURATION; MYOTUBULARIN; MACROPHAGES; BIOGENESIS;

D O I：

10.1016/j.febslet.2012.09.043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PtdIns(3) P (phosphatidylinositol 3-phosphate) is a signaling molecule important for phagosome maturation. The major role of Vps34 in production of phagosomal PtdIns(3) P has been indicated. However, the fate of the newly generated PtdIns(3) P has not been well described. Here we show that elimination of PtdIns(3) P from phagosomal membrane was significantly delayed in RAW264.7 macrophages lacking PTEN or PIKfyve. In the PTEN-deficient cells treated with a PIKfyve inhibitor, degradation of PtdIns(3) P was almost lost, indicating that PTEN and PIKfyve are two major players in phagosomal PtdIns(3) P metabolism. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4010 / 4015

页数：6

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