Waveguide-modulated surface plasmon-coupled emission of Nile blue in poly(vinyl alcohol) thin films

被引:36
作者
Gryczynski, Ignacy [1 ]
Malicka, Joanna [1 ]
Nowaczyk, Kazimierz [1 ]
Gryczynski, Zygmunt [1 ]
Lakowicz, Joseph R. [1 ]
机构
[1] Univ Maryland, Ctr Fluorescence Spect, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA
关键词
surface plasmon-coupled emission; waveguide; silver film; Nile Blue;
D O I
10.1016/j.tsf.2005.07.312
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
Surface plasmon-coupled emission (SPCE) phenomenon is the coupling of excited fluorophores near a silver film with surface plasmons, resulting in directional emission into the underlying glass substrates. We report a complex coupling of Nile Blue fluorophore with 50 nm silver mirror, resulting in emission at several angles in the glass substrate, with either s or p polarization. This complex pattern of directional and polarized emission appears to be due to optical waveguide effects occurring when the sample thickness becomes comparable to the emission wavelength. We expect waveguide-modulated SPCE to have applications to biophysics and sensing. (c) 2006 Published by Elsevier B.V.
引用
收藏
页码:15 / 20
页数:6
相关论文
共 27 条
[21]   A commercial solution for surface plasmon sensing [J].
Melendez, J ;
Carr, R ;
Bartholomew, DU ;
Kukanskis, K ;
Elkind, J ;
Yee, S ;
Furlong, C ;
Woodbury, R .
SENSORS AND ACTUATORS B-CHEMICAL, 1996, 35 (1-3) :212-216
[22]  
Okamoto K., 2021, Fundamentals of Optical Waveguides
[23]  
Raether H., 1977, PHYS THIN FILMS, V9, P145
[24]  
Raether H., 1988, SURFACE PLASMONS SMO
[25]   Coupled plasmon-waveguide resonators: A new spectroscopic tool for probing proteolipid film structure and properties [J].
Salamon, Z ;
Macleod, HA ;
Tollin, G .
BIOPHYSICAL JOURNAL, 1997, 73 (05) :2791-2797
[26]   Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein systems .1. Theoretical principles [J].
Salamon, Z ;
Macleod, HA ;
Tollin, G .
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON BIOMEMBRANES, 1997, 1331 (02) :117-129
[27]   Attomolar sensitivity in bioassays based on surface plasmon fluorescence spectroscopy [J].
Yu, F ;
Persson, B ;
Löfäs, S ;
Knoll, W .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (29) :8902-8903