Proteomics in Rapeseed Microspore Embryogenesis

被引：6

作者：

Cordewener, J. ^{[1
]}

van der Wal, F. ^{[1
]}

Joosen, R. ^{[1
]}

Boutilier, K. ^{[1
]}

America, T. ^{[1
]}

机构：

[1] Plant Res Int, NL-6700 AA Wageningen, Netherlands

来源：

ADVANCES IN HAPLOID PRODUCTION IN HIGHER PLANTS | 2009年

关键词：

Rapeseed (Brassica napus); microspores; proteomics; 2-D gel electrophoresis; DiGE; phosphorylation; BRASSICA-NAPUS L; PROTEIN-SYNTHESIS; HEAT-SHOCK; CV TOPAS; INDUCTION; POLLEN; IDENTIFICATION; CULTURES; DENSITY;

D O I：

10.1007/978-1-4020-8854-4_10

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

This chapter reviews the proteomics research performed to elucidate the molecular processes associated with embryo initiation and development in Brassica napus (rapeseed) microspore-derived embryo cultures. Classical biochemical methods such as combined [(35)S]-methionine labelling and 2-D gel electrophoresis (2-DE) have been used for the large-scale study of proteins differentially expressed during the inductive phase of embryogenesis. Immuno-cytological experiments have been carried out to study the (sub)cellular localization of putative marker proteins for embryo induction. Changes in post-translational modification of proteins (phosphorylation) have been detected during the induction phase, as have changes in the profile of proteins en peptides secreted by developing embryos. We also discuss recent developments in quantitative proteomics technologies such as fluorescence labelling of protein samples and stable isotope labelling by amino acids in cell cultures that enable more detailed expression analyses and the identification of low abundant proteins in complex mixtures.

引用

页码：135 / 146

页数：12

共 21 条

[1] A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. [J].