Generation of induced pluripotent mouse stem cells in an indirect co-culture system

被引：1

作者：

Dong, F. L. ^{[1
,3
,4
]}

Kaleri, H. A. ^{[2
]}

Lu, Y. D. ^{[1
]}

Song, C. L. ^{[1
]}

Jiang, B. C. ^{[1
]}

Zhang, B. L. ^{[1
]}

Wang, L. J. ^{[1
]}

Wang, X. G. ^{[1
]}

Ma, X. S. ^{[1
]}

Wu, B. J. ^{[5
]}

Song, H. ^{[6
]}

Li, J. ^{[1
]}

Liu, H. L. ^{[1
]}

机构：

[1] Nanjing Agr Univ, Dept Anim Breeding & Genet, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China

[2] Sindh Agr Univ, Dept Anim Breeding & Genet, Fac Anim Husb & Vet Sci, Tandojam, Pakistan

[3] Soochow Univ, Inst Biol, Suzhou, Peoples R China

[4] Soochow Univ, Inst Med Sci, Suzhou, Peoples R China

[5] Inner Mongolia Saikexing Reprod Biotechnol Co Ltd, Hohhot, Peoples R China

[6] Nanjing Agr Univ, Ctr Anim Embryo Engn & Technol, Nanjing, Jiangsu, Peoples R China

来源：

GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 04期

关键词：

Induced pluripotent stem cells; Feeder cells; Co-culture; Microporous polymer membrane; GROWTH;

D O I：

10.4238/2012.December.6.1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Typically, production of induced pluripotent stem cells requires direct contact with feeder cells. However, once the stem cells have reached the appropriate maturation point, it is difficult to separate them from feeder cells, which must be irradiated with gamma-rays or treated with the antibiotic mitomycin-C. We used a microporous poly-membrane-based indirect contact co-culture system with mouse embryonic fibroblasts to induce mouse pluripotent stem cells without radiation or antibiotics. We found that induced pluripotent stem cells induced by this co-culture method had a reprogramming efficiency and time similar to those induced using traditional methods. Furthermore, strongly expressed pluripotent markers showed a normal karyotype and formation and contained all three germ layers in a teratoma.

引用

页码：4179 / 4186

页数：8

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